Phytochemical profile for Cestrum nocturnum leaves ethanolic extract and isolation of a rare flavonoid using different chromatographic and spectroscopic techniques

Abstract

In this study, flavonoids and phenolic acids were detected in the alcoholic extract of Cestrum nocturnum leaves using chromatographic technique. Phytochemical survey was also conducted for the different parts of the plant using more than one experiment for each component we wanted to search for. A quantitative estimate was also made for the content of plant leaves from secondary metabolites, specifically alkaloids, flavonoids, saponins, tannins and phenolic acids. All experiments showed that the leaves of the Cestrum nocturnum plant are rich in many phenolic compounds and flavonoids, which prompted us to separate one of these compounds by traditional separation methods. The results showed that the leaves of the Cestrum nocturnum plant are rich in secondary metabolites in different fractions and Phytochemical profiles using HPLC (high-performance liquid chromatography) explain, leaves of the plant are expensive with lots of chemical compounds, like cinnamic acid vanillin, gallic acid, luteolin, kaempferol, caffeic acid, quercetin, propyl gallate, isovetixin, rutin, narinjirnin, coumaric acid, cinnamic acid, apigenin, chlorogenic acid, and hispertin. Quantitative estimation of total phytochemicals main groups for different plant parts exhibits that all parts of the plant contain the main groups of secondary metabolites (flavonoids, alkaloids, saponin, and tannins) in good quantities, especially the leaves. The propyl gallate compound recovered from 96 percent ethanolic leaves extract using different chromatographic methods was then identified using Rf-values, UV, 1H-NMR spectrum studies, and mass spectroscopy.