Development and quality evaluation of beetroot-based functional ice cream

Introduction

The global food industry has undergone a significant transformation in recent years, shifting from conventional calorie-dense products to functional foods that offer additional health benefits beyond basic nutrition. This paradigm shift is largely driven by increasing consumer awareness regarding diet-related chronic diseases, including cardiovascular disorders, diabetes, obesity, and certain cancers. As a result, there is growing demand for foods enriched with bioactive compounds such as antioxidants, dietary fiber, vitamins, and phytochemicals. Among these, functional dairy products, particularly ice cream, have emerged as promising carriers for delivering health-promoting ingredients due to their widespread acceptability across different age groups. Traditionally, ice cream is considered an indulgent frozen dessert characterized by high fat and sugar content. However, modern advancements in food science and technology have facilitated its transformation into a functional multiphase delivery system, capable of incorporating nutritionally valuable ingredients without compromising sensory appeal. The integration of plant-based bioactive components into ice cream formulations has gained considerable attention, as it not only enhances nutritional value but also aligns with the growing preference for clean-label and natural food products. In this context, beetroot (Beta vulgaris) has attracted significant interest as a functional ingredient due to its exceptional nutritional and therapeutic properties. Beetroot is a root vegetable belonging to the Amaranthaceae family and is widely cultivated across temperate regions. It is rich in unique bioactive compounds, particularly betalains, which are nitrogencontaining pigments responsible for its characteristic red-purple color. Betalains are classified into betacyanins (red pigments) and betaxanthins (yellow pigments), both of which exhibit strong antioxidant activity. These compounds have been extensively studied for their potential role in reducing oxidative stress, inflammation, and the risk of chronic diseases (Clifford et al., 2015; Sadowska-Bartosz & Bartosz, 2021) [2, 13] . In addition to betalains, beetroot is an excellent source of dietary nitrates, which play a crucial role in cardiovascular health by improving endothelial function and enhancing nitric oxide bioavailability. Regular consumption of beetroot has been associated with reduced Blood pressure, improved exercise performance, and enhanced metabolic efficiency. Furthermore, beetroot contains significant amounts of phenolic compounds, vitamins (such as vitamin C and folate), minerals (including potassium and iron), and dietary fiber, all of which contribute to its functional potential. Recent trends in food product development emphasize the use of natural colorants as alternatives to synthetic additives, which have been associated with potential health risks. In this regard, beetroot serves as an excellent natural coloring agent, providing an attractive red hue while simultaneously enhancing the nutritional profile of food products. This dual functionality makes beetroot particularly suitable for incorporation into frozen desserts like ice cream, where visual appeal plays a critical role in consumer acceptance. Despite its numerous advantages, the incorporation of beetroot into ice cream systems presents several technological challenges. The stability of betalains is highly sensitive to environmental factors such as temperature, pH, oxygen, and light exposure. Processing conditions, including pasteurization, homogenization, and freezing, can significantly influence the retention of these bioactive compounds. Additionally, the presence of dietary fiber and plant solids can alter the physicochemical properties of ice cream, affecting parameters such as viscosity, overrun, texture, and melting behavior. Another important consideration is the sensory impact of beetroot incorporation. While it enhances color and nutritional value, beetroot may impart a characteristic earthy flavor that can influence consumer acceptability. Therefore, optimizing formulation parameters, including the level of beetroot incorporation and the use of flavor-masking agents, is essential to achieve a balance between functionality and sensory quality. The development of beetroot-based functional ice cream also aligns with the broader objectives of sustainable food systems and waste valorization. Beetroot processing generates by-products such as peels and pulp residues, which are rich in bioactive compounds and can be effectively utilized in food formulations. This approach not only enhances the economic value of agricultural produce but also contributes to reducing food waste and promoting environmental sustainability. In recent years, advancements in microstructure engineering and food processing technologies have opened new avenues for improving the stability and functionality of bioactive compounds in frozen desserts. Techniques such as microencapsulation, high-pressure homogenization, and controlled freezing have been explored to enhance the retention of sensitive compounds like betalains and to improve the overall quality of functional ice cream. Therefore, the development and quality evaluation of beetroot-based functional ice cream represent a promising area of research that integrates nutritional science, food technology, and consumer-oriented product development. This review aims to provide a comprehensive overview of the role of beetroot as a functional ingredient in ice cream, focusing on its nutritional significance, effects on physicochemical and sensory properties, processing challenges, and future research prospects.

Ice cream as a functional multiphase system

Ice cream is a complex multiphase system consisting of ice crystals, air cells, fat globules, and an unfrozen serum phase. The quality of ice cream depends on the interaction among these phases.

Incorporation of beetroot modifies this structure by: • Increasing total solids and dietary fiber • Enhancing viscosity and water-binding capacity • Reducing overrun due to restricted air incorporation Studies indicate that vegetable-based additives reduce overrun but improve melting resistance and structural stability (Muse & Hartel, 2004) [7] . Beetroot fiber and pectin contribute to improved rheological properties, making it a natural stabilizer in ice cream formulations.

Nutritional and functional potential of beetroot

Beetroot is a rich source of bioactive compounds:

• Betalains (betanin, vulgaxanthin): Strong antioxidants • Dietary nitrates: Improve cardiovascular health and blood flow • Phenolic compounds: Provide anti-inflammatory benefits • Dietary fiber: Supports gut health Research shows that beetroot supplementation significantly increases total antioxidant capacity in dairy products (Georgiev et al., 2010) [4] .

Additionally, beetroot contributes:

• Natural red-purple color (clean-label advantage) • Reduced need for artificial additives • Improved nutritional density

Impact of Processing on Beetroot Bioactives

Processing conditions play a critical role in maintaining beetroot quality: Thermal Processing • Moderate heating enhances extractability of betalains • Excess heat causes degradation and color loss

Freezing and Storage

• Freezing preserves nutrients but may disrupt cell structure • Betalains degrade over time due to oxidation and light exposure Studies report a significant reduction in betalain content during prolonged storage, highlighting the need for protective strategies such as encapsulation (Azeredo, 2009) [

Formulation Challenges in Beetroot Ice

References

1. Azeredo HMC. Betalains: Properties, sources, applications, and stability-A review. International Journal of Food Science & Technology. 2009;44(12):2365-2376. doi:10.1111/j.13652621.2007.01668.x
2. Clifford T, Howatson G, West DJ, Stevenson EJ. The potential benefits of red beetroot supplementation in health and disease. Nutrients. 2015;7(4):2801-2822. doi:10.3390/nu7042801
3. El-Sayed SM, Youssef AM, El-Sayed HS. Innovative dairy-based products featuring vegetables: A complementary technique with promising perspectives. Journal of Food Measurement and Characterization. 2025;19(11):8165-8181.
4. Georgiev VG, Weber J, Kneschke EM. Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot (Beta vulgaris cv. Detroit dark red). Food Chemistry. 2010;119(2):508-515. doi:10.1016/j.foodchem.2009.06.033
5. Hassan MF, Barakat H. Effect of carrot and pumpkin pulps addition on chemical, rheological, nutritional and organoleptic properties of ice cream. Food and Nutrition Sciences. 2018;9(8):969-982. doi:10.4236/fns.2018.98071
6. Khan MI. Stabilization of betalains: A review. Food Chemistry. 2016;197:1280-1285. doi:10.1016/j.foodchem.2015.11.043
7. Muse MR, Hartel RW. Ice cream structural elements that affect melting rate and hardness. Journal of Dairy Science. 2004;87(1):1-10. doi:10.3168/jds.S00220302(04)73135-5
8. Ninčević Grassino A, et al. Carotenoid content and profiles of pumpkin products and by-products. Molecules. 2023;28(2):858. doi:10.3390/molecules28020858
9. Patel AS, Bariya AR, Ghodasara SN, Chavda JA, Patil SS. Total carotene content and quality characteristics of pumpkin flavoured buffalo milk. Heliyon. 2020;6(7):e04379. doi:10.1016/j.heliyon.2020.e04379
10. Peasura N, Sinchaipanit P, Sangsuriyawong A, Disnil S. Effect of pumpkin on quality, nutritional and organoleptic properties of ice cream. Agriculture and Natural Resources. 2020;54(5):521-528.
11. Pitalua E, Jimenez M, Vernon-Carter EJ, Beristain CI. Antioxidative activity of microcapsules with beetroot juice using gum Arabic as wall material. Food Chemistry. 2010;122(3):927-936. doi:10.1016/j.foodchem.2010.03.036
12. Sabharwal P, Thakur S, Shrestha S, Fu Y. Breeding and genetics of resistance to major diseases in Cucurbita: A review. Crop Science. 2024. Advance online publication.
13. Sadowska-Bartosz I, Bartosz G. Biological properties and applications of betalains. Molecules. 2021;26(9):2520. doi:10.3390/molecules26092520
14. Singh B, Hathan BS, Kaur A. Development of functional ice cream incorporating vegetable pulp. Journal of Food Processing and Preservation. 2022;46(5):e16521. doi:10.1111/jfpp.16521
15. Szydłowska A, Zielińska D, Kołożyn-Krajewska D. Effect of pumpkin cultivar on selected quality parameters of functional non-dairy frozen desserts. Applied Sciences. 2022;12(16):8063. doi:10.3390/app12168063