Optimized protocol for DNA extraction from ancient skeletal human remains using Chelex-100

Abstract

PCR-based analysis of skeletonized human remains is a common aspect in both forensic human identification as well as Ancient DNA research. In this, both areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality of recovered DNA and presence of inhibitory substances in samples from excavated remains. To enable amplification based analysis of the remains, development of optimized DNA extraction procedures is thus a critical factor in both areas.The study here presents an optimized protocol for DNA extraction from ancient skeletonized remains using Chelex-100, which proved to be effective in yielding amplifiable extracts from sample material excavated after centuries in a soil environment, which consequently have high inhibitor content and overall limited DNA preservation. Success of the optimization strategies utilized is shown in significantly improved amplification outcomes compared to the predecessor method