Flow cytometric detection of CD79a expression in DMBA induced mammary tumour treated with withaferin a in female Sprague-Dawley rats

Abstract

The present study was designed to examine the expression of CD79a, total B lymphocytes in DMBA (7,12-dimethylbenz[a]anthracene) induced rat mammary carcinogenesis by flow cytometry. Twenty four female Sprague-Dawley (SD) rats were equally distributed to control, DMBA, DMBA+Tamoxifen (Standard drug) and DMBA+Withaferin A groups. Peripheral blood was collected at the 120th day from the initial dose of DMBA and CD79a, total B lymphocytes were determined by using flow cytometry. There was a significant increase in the total B lymphocytes (CD79a) in the DMBA+Withaferin A group from the DMBA+Tamoxifen group indicating the immunostimulatory effect of Withaferin A.