Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia

Abstract

A Gram-positive, motile, short-rod-shaped strain, designated YIM 004(T), was isolated from a forest-soil sample collected from Lijiang, Yunnan Province, China, and was investigated using a polyphasic taxonomic approach. The isolate contained chemotaxonomic markers that corresponded to those of its phylogenetic neighbour, Georgenia muralis, i.e. it possessed peptidoglycan type A4 alpha with lysine as the diagnostic cell-wall diamino acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acid was ai-C(15 : 0). The G+C content of the genomic DNA was 72.9 mol%. Strain YIM 004(T) exhibited a 16S rRNA gene sequence similarity of 97.3 % and a DNA-DNA relatedness value of 18 % with respect to G. muralis DSM 14418(T). On the basis of the phenotypic and genotypic differences between the isolate and G. muralis, strain YIM 004(T) represents a novel species of the genus Georgenia, for which the name Georgenia ruanii sp. nov. is proposed. The type strain is YIM 004(T) (=CCTCC AB 204065(T)=DSM 17458(T)=KCTC 19029(T)). In addition, an emended description of the genus Georgenia is presented.