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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Analytical Biochemistry · 1983 · Vol. 132(1) · pp. 6–13
Andrew P. Feinberg✉(Johns Hopkins Medicine)Bert Vogelstein(Johns Hopkins University)
Advanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryParasitic Infections and DiagnosticsEndonucleaseDNARestriction enzymeAgaroseOligonucleotideChemistryMolecular biologyDNA polymeraseDNA clampPolymerase
MeSH terms
AutoradiographyBase SequenceAnimalsCytidine TriphosphateDNADNA Restriction EnzymesElectrophoresis, Agar GelHumansPhosphorus RadioisotopesPlasmidsTemplates, Genetic
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References
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Nucleic Acids Research · 1979 · 14,865 citations
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
Journal of Molecular Biology · 1977 · 11,660 citations
Enhanced autoradiographic detection of <sup>32</sup>P and <sup>125</sup>I using intensifying screens and hypersensitized film
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Detection of specific sequences among DNA fragments separated by gel electrophoresis
Journal of Molecular Biology · 1975 · 32,825 citations
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