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Effect of calcium nitrate and PDKV micro-grade II on growth, yield and quality of gladiolus

International Journal of Advanced Biochemistry Research · 2026 · Vol. 10(8S) · pp. 555–558

Abstract

A field experiment entitled “Effect of calcium nitrate and PDKV micro-grade II on growth, yield and quality of gladiolus” was conducted during the rabi season of 2025-26 at Satpuda Botanic Garden, College of Agriculture, Nagpur (M.S.). The experiment was conducted to find out the influence of foliar application of calcium nitrate and PDKV micro-grade II on the growth, flowering, yield and quality of gladiolus and to find out the best treatment combination. The experiment was conducted in Factorial Randomized Block Design with sixteen treatment combination of four levels of calcium nitrate (0, 0.5, 1.0 and 1.5%) and four levels of PDKV micro-grade II (0, 0.5, 1.0 and 1.5%). The treatment combination of 1.0% calcium nitrate + 1.5% PDKV micro-grade II (C₃M₄) recorded maximum plant height, spike length and florets per spike indicating its superiority for vegetative growth and spike yield. The application of 1.5% calcium nitrate (C₄) and 1.5% PDKV micro-grade II (M₄) significantly improved leaf area, spikes per plant, vase life, corms per plant and cormels per plant. Earliness in flowering was observed with 1.5% calcium nitrate (C₄) and 1.0% PDKV micro-grade II (M₃). Overall, the combination of 1.0% calcium nitrate + 1.5% PDKV micro-grade II was found most effective for enhancing growth and spike quality, whereas 1.5% calcium nitrate and 1.5% PDKV micro-grade II proved superior for improving corm and cormel yield.

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Introduction

Gladiolus (Gladiolus grandiflorus L.), popularly known as the "Queen of Bulbous Flower Crops," is one of the most important commercial cut flowers in India. Its attractive spikes, wide colour range, long vase life, and excellent keeping quality make it highly desirable for bouquets, floral arrangements, and landscaping. With increasing domestic and export demand, gladiolus has emerged as a profitable floriculture crop contributing significantly to India's cut flower industry. The production of superior-quality spikes depends on balanced nutrient management. While macronutrients drive vegetative growth, calcium and micronutrients are crucial for enhancing spike strength, floret quality, corm development, and post-harvest longevity. Foliar application of calcium nitrate supplies readily available calcium and nitrate, improving cell wall stability, nutrient uptake, and delaying senescence. Similarly, micronutrients such as iron, zinc, boron, manganese, copper, and molybdenum are indispensable for chlorophyll synthesis, enzyme activation, photosynthesis, pollen germination, and protein metabolism. Deficiencies in these elements directly reduce growth, flowering, and yield. Considering the rising demand for premium gladiolus flowers, foliar nutrition offers an efficient approach to improve nutrient use efficiency and flower quality. The combined application of calcium nitrate and PDKV micro-grade II is expected to enhance growth, spike yield, corm production, and overall quality attributes. Therefore, the present investigation entitled "Effect of calcium nitrate and PDKV micro-grade II on growth, yield and quality of gladiolus" was undertaken to evaluate different concentrations and identify the most suitable combination for achieving superior crop performance.

Materials and Methods

The experiment was conducted during the winter season of 2025-26 at the Satpuda Botanic Garden, College of Agriculture, Nagpur, Maharashtra, to evaluate the effect of calcium nitrate and PDKV Micro-grade II on the growth, flowering, yield and quality of gladiolus (Gladiolus grandiflorus L.) cv. PDKV Satpuda Purple. The trial was laid out in a factorial randomized block design (FRBD) comprising sixteen treatment combinations with three replications. Uniform, healthy corms (4-5 cm diameter) were treated with 0.3% Captan before planting on ridges and furrows at a spacing of 45 cm × 15 cm. The recommended fertilizer dose of 300:200:200 kg N:P₂O₅: K₂O ha -1 was applied and foliar sprays of calcium nitrate (0, 0.5, 1.0 and 1.5%) and PDKV Micro-grade II (0, 0.5, 1.0 and 1.5%) were given twice at the four-leaf and six-leaf stages. Standard agronomic practices, including irrigation, weeding, earthing-up, staking and plant protection, were followed throughout the crop growth period. Five randomly selected plants from each plot were used to record observations on growth, flowering, spike quality, vase life, corm and cormel yield. Statistical analysis of the recorded data was carried out following the methodology proposed by Panse and Sukhatme (1967) [11] . [10] and Nagamani et al. (2017) [8] in gladiolus.

Results and Discussion

b. Flowering parameters

The [10] , Nagamani et al. (2017) [8] in gladiolus and Abood (2017) [2] in Tulipa sp.

c. Corm yield and quality parameters

The highest number of corms per plant (2.73) was recorded under 1.5% concentrations (C 4 ), at par with 1.0% (C 3 , 2.68).

Similarly, maximum cormels per plant were obtained with 1.5% calcium nitrate (C 4 , 21.17), statistically at par with 1.0% (C 3 , 21.03). The improvement in yield attributes is attributed to calcium and nitrogen promoting cell division, cell wall rigidity, biomass distribution, chlorophyll synthesis, and photosynthetic product accumulation. These processes strengthen source-sink relationships, enhance starch synthesis, and support daughter cormel formation.

Comparable results in gladiolus were reported by Qureshi et al. (2015) [12] , Reddy and Sarkar (2016) [13] , and Ahmad and Rab (2020) [3] .

Effect of PDKV micro-grade II a. Growth parameters

Maximum plant height (82.03 cm) was recorded under 1.5% concentration (M 4 ), followed by 1.0% concentration (M 3 , 77.00 cm). Leaf area was also highest in 1.5% concentration (M 4 , 106.73 cm²), statistically at par with M 3 i.e. 1.0% (104.10 cm²). The improvement in growth parameters may be attributed to zinc promoting tryptophan synthesis for auxin production and nitrogen metabolism, along with boron, calcium, and magnesium enhancing cell division, enlargement, and chlorophyll synthesis. Similar findings were reported by Abid Kamal et al. (2022) [1] and Harish et al. (2023) [5] in gladiolus.

b. Flowering parameter

The (2013) [14] , Katiyar et al. (2005) [7] , Fahad et al. (2014) [4] , Naik et al. (2015) [9] , Kashyap and Tikey (2022) [6] , and Harish et al. (2023) [5] in gladiolus.

c. Corm yield and quality parameters

The highest number of corms per plant (2.78) was recorded under 1.5% concentration (M 4 ), followed by 1.0% (M 3 , 2.65). Similarly, maximum cormels per plant were obtained with M 4 i.e. 1.5% concentration (21.33), statistically at par with M 3 i.e. 1.0% concentration (20.70). The observed increase in yield attributes may be attributed to adequate micronutrient supply through foliar application. Zinc plays a key role in enzyme activity, auxin and cytokinin synthesis, and balancing phosphorus and potassium uptake. In association with other micronutrients, zinc improves chlorophyll function, carbohydrate partitioning, and dry matter translocation towards corm and cormel development. These findings were in line with Singh et al. (2012) [15] , Fahad et al. (2014) [4] , and Kashyap and Tikey (2022) [6] .

Interaction effect

The interaction between calcium nitrate and PDKV micrograde II levels was significant for growth and quality traits in gladiolus ( [7] , supporting the present findings.

Conclusion

From the present experimental trial, it is concluded that the maximum vegetative growth, flower yield, quality parameters and corm attributes were recorded under 1.5% concentration of calcium nitrate and 1.5% concentration of PDKV micro-grade II. The treatment combination of 1.0% calcium nitrate + 1.5% PDKV micro-grade II (C 3 M 4 ) was found to be superior in respect plant height, spike length and number of florets spike -1 .

References

  1. Abid Kamal M, Rahman M, Gul G, Khan FU, Bashir A, Naeem A, et al. A review on quality, growth and yield parameters of gladiolus as affected by micronutrients of foliar application. J Xi'an Shiyou Univ Nat Sci Edition. 2022;18(8):720-730.
  2. Abood B. Effect of bio-fertilizer and calcium nitrate on vegetative and flowering growth of Tulipa sp. cv. Orange Casing. In: Proceedings of the 5th International Conference on Chemical, Biological, Agricultural and Environmental Sciences; 2017 Apr 28-30; Kuala Lumpur, Malaysia. p. 25-30.
  3. Ahmad M, Rab A. Calcium effects on post-harvest attributes and vase life of gladiolus using different methods of application. Pak J Bot. 2020;52(1):167-179.
  4. Fahad S, Ahmad M, Anjum A, Hussain S. The effect of micronutrients (B, Zn and Fe) foliar application on the growth, flowering and corm production of gladiolus (Gladiolus grandiflorus L.) in calcareous soils. J Agric Sci Tech. 2014;16(7):1671-1682.
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  7. Katiyar RS, Garg VK, Singh PK. Foliar spray of Zn and Cu on growth, floral characteristics and yield of gladiolus grown in sodic soil. Indian J Hortic. 2005;62(3):272-275.
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  9. Naik DV, Dhaduk BK, Kapadia DB, Jambhale SS. Studies on the effect of different micronutrients in gladiolus cv. American Beauty. Ecol Environ Conserv. 2015;21(Suppl):425-428.
  10. Padmalatha T, Reddy GS, Chandrasekhar R, Shankar AS, Chaturvedi A. Effect of foliar sprays of bioregulators on growth and flowering in gladiolus plants. Progr Hortic. 2014;46(2):289-292.
  11. Panse VG, Sukhatme PV. Statistical methods for agricultural workers. 2nd ed. New Delhi: Indian Council of Agricultural Research; 1967. 381 p.
  12. Qureshi A, Qadri ZA, Nazki IT. Influence of calcium nitrate [Ca(NO₃)₂] sprays and cormel grades on vegetative growth and corm production in gladiolus (Gladiolus hybridus Hort.) cv. Priscilla. J Ornam Hortic. 2015;18(4):126-134.
  13. Reddy A, Sarkar MM. Studies on the effect of foliar application of calcium on post-harvest, corm and cormel production in gladiolus cv. Summer Sunshine. Int J Agric Environ Biotechnol. 2016;9(1):89-94.
  14. Sharma J, Gupta AK, Kumar C, Gautam RKS. Influence of zinc, calcium and boron on vegetative and flowering parameters of gladiolus cv. Aldebran. The Bioscan. 2013;8(4):1153-1158.
  15. Singh JP, Kumar K, Katiyar PN. Effect of zinc, iron and copper on yield parameters of gladiolus. HortFlora Res Spectrum. 2012;1(1):64-68.
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