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Response of mustard (Brassica juncea L.) to different levels of sulphur and zinc on yield and oil content

International Journal of Agriculture and Food Science · 2026 · Vol. 8(5) · pp. 482–492
Ishika ParaliyaShivam YadavRohitashv NagarSHIVENDRA P. SINGHGunnjeet KaurHimanshu Jain

Abstract

A field experiment was conducted during the Rabi season of 2025-26 at the Instructional Farm, Career Point University, Kota (Rajasthan), to evaluate the effect of different levels of sulphur and zinc on growth, yield, and quality of mustard. The experiment consisted of 16 treatment combinations with four levels of sulphur (0, 10, 20, and 30 kg ha⁻¹) and four levels of zinc (0, 5, 10, and 15 kg ha⁻¹) arranged in Factorial Randomized Block Design with three replications. Results revealed that combined application of sulphur and zinc significantly improved growth parameters, yield attributes, and yield. The highest number of siliquae per plant (277.4), siliqua length (5.6 cm), and improved growth were recorded under S₃Z₃ (30 kg S + 15 kg Zn ha⁻¹). However, maximum seed yield (24.2 q ha⁻¹) was observed under S₁Z₀ (10 kg S ha⁻¹) and S₃Z₂ (30 kg S + 10 kg Zn ha⁻¹). The study concluded that balanced application of sulphur and zinc enhances productivity and quality of mustard.

Full Text

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Introduction

Mustard is one of the major oilseed crops in India, contributing significantly to edible oil production. However, its productivity remains low due to imbalanced fertilization and micronutrient deficiencies. Sulphur plays a vital role in oil synthesis and protein formation, while zinc is essential for enzyme activation, auxin synthesis, and reproductive development. Deficiency of these nutrients leads to reduced growth, poor seed formation, and low yield. The combined application of sulphur and zinc has been reported to improve growth, yield, and oil content due to their synergistic interaction. Therefore, the present study was conducted to evaluate their effect on mustard productivity. SEm± 0.70 CD (P = 0.05) NS However, numerically higher plant population (14.2 plants m⁻²) was recorded under T₃ (0 kg S + 10 kg Zn ha⁻¹), T₆ (10 kg S + 5 kg Zn ha⁻¹), T₁₁ (20 kg S + 10 kg Zn ha⁻¹), T₁₂ (20 kg S + 15 kg Zn ha⁻¹), T₁₃ (30 kg S + 0 kg Zn ha⁻¹), T₁₄ (30 kg S + 5 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹).

Although the lowest value (13.9 plants m⁻²) was observed under control (T₁), all treatments were found statistically at par.

Data pertaining to plant height at different growth stages indicated significant variation due to treatment combinations. At 20 DAS, maximum plant height (18.4 cm) was recorded under T₃ (0 kg S + 10 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹), which were at par with T₁₂ (20 kg S + 15 kg Zn ha⁻¹) and T₁₆ (30 kg S + 15 kg Zn ha⁻¹). However, the lowest plant height (12.2 cm) was recorded under control (T₁).

Yield attributes

Data pertaining to yield attributes viz., number of siliquae plant⁻¹, siliqua length (cm), number of seeds siliqua⁻¹ and test weight (g) as influenced by different levels of sulphur and zinc are presented below.

Number of siliquae plant⁻¹

Data revealed that the number of siliquae plant⁻¹ was significantly influenced by different treatment combinations.

The maximum number of siliquae plant⁻¹ (277.4) was recorded under T₁₆ (30 kg S + 15 kg Zn ha⁻¹), which was significantly superior over most of the treatments; however, it remained at par with T₁₄ (30 kg S + 5 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹).

Although T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₄ (0 kg S + 15 kg Zn ha⁻¹) also recorded comparatively higher values (256.2 and 254.8, respectively), these were at par with several other treatments. The lowest number of siliquae plant⁻¹ (201.9) was recorded under control (T₁: 0 kg S + 0 kg Zn ha⁻¹). Moreover, treatments receiving higher levels of sulphur in combination with zinc showed a consistent increase in siliquae formation, indicating better reproductive growth.

Data pertaining to siliqua length indicated significant variation among treatments. The maximum siliqua length (5.6 cm) was recorded under T₁₆ (30 kg S + 15 kg Zn ha⁻¹), which was significantly superior; however, it was found at par with T₃ (0 kg S + 10 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹). Although T₂ (0 kg S + 5 kg Zn ha⁻¹) and T₁₄ (30 kg S + 5 kg Zn ha⁻¹) also recorded higher siliqua length (5.0 and 4.9 cm), these were statistically at par with many treatments. The minimum siliqua length (3.6 cm) was observed under T₄ (0 kg S + 15 kg Zn ha⁻¹). Thus, it is evident that combined application of sulphur and zinc improved siliqua development; however, differences among treatments were not always statistically distinct.

Number of seeds siliqua⁻¹

Data revealed that the number of seeds per siliqua did not vary significantly due to different treatments. The values ranged from 11.6 to 12.3. However, numerically higher number of seeds siliqua⁻¹ (12.3) was recorded under T₂ (0 kg S + 5 kg Zn ha⁻¹), T₃ (0 kg S + 10 kg Zn ha⁻¹), T₁₀ (20 kg S + 5 kg Zn ha⁻¹) and T₁₆ (30 kg S + 15 kg Zn ha⁻¹).

Although the lowest value (11.6) was recorded under control (T₁), all treatments were statistically at par. Moreover, this indicates that seeds per siliqua is a genetically stable trait and less influenced by nutrient levels.

Data pertaining to seed yield, stover yield, biological yield and harvest index as influenced by different levels of sulphur and zinc are presented below.

Seed Yield (q ha⁻¹) Data revealed that seed yield was significantly influenced by different treatment combinations. The maximum seed yield (24.2 q ha⁻¹) was recorded under T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹), which were significantly superior over most of the treatments; however, these were found at par with T₃ (0 kg S + 10 kg Zn ha⁻¹) and T₁₃ (30 kg S + 0 kg Zn ha⁻¹). Although T₁₂ (20 kg S + 15 kg Zn ha⁻¹) also produced higher seed yield (21.7 q ha⁻¹), it remained at par with T₄ and T₉. The lowest seed yield (12.6 q ha⁻¹) was recorded under control (T₁: 0 kg S + 0 kg Zn ha⁻¹). Moreover, the increase in seed yield with sulphur and zinc application may be attributed to improved growth and yield attributes; however, several treatments were statistically at par, indicating marginal differences at higher levels.

Stover yield (q ha⁻¹) Data pertaining to stover yield showed significant variation among treatments. The maximum stover yield (110.4 q ha⁻¹) was recorded under T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹), which were significantly superior; however, these were at par with T₃ (101.7 q ha⁻¹) and T₁₃ (101.7 q ha⁻¹).

Although T₁₂ and T₉ also recorded higher stover yield, they were statistically at par with several treatments. The lowest stover yield (53.9 q ha⁻¹) was observed under control (T₁).

Biological yield (q ha⁻¹) Data revealed that biological yield followed a similar trend as seed and stover yield. The maximum biological yield (134.5 q ha⁻¹) was recorded under T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹), which were significantly superior over control; however, these were at par with T₃ and T₁₃.

Although T₁₂ (121.2 q ha⁻¹) also produced higher biological yield, it remained at par with several treatments. The lowest biological yield (66.5 q ha⁻¹) was recorded under control (T₁).

Data pertaining to harvest index indicated non-significant variation among treatments. The values ranged from 17.4 to 21.2%. However, numerically higher harvest index (21.2%) was recorded under T₂ (0 kg S + 5 kg Zn ha⁻¹), followed by T₆ (20.8%) and T₁₆ (20.8%). Although control recorded lower harvest index (19.0%), all treatments were found statistically at par. Overall, data indicated that application of sulphur and zinc significantly improved seed, stover and biological yield. However, harvest index did not vary significantly and remained at par among treatments. Moreover, treatments T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹) proved superior in terms of yield, although these were statistically at par with T₃ and T₁₃.

Quality parameters

Data pertaining to quality parameters viz., oil content (%) and oil yield (kg ha⁻¹) as influenced by different levels of sulphur and zinc are presented below.

Data revealed that oil content was not significantly influenced by different treatment combinations. The oil content ranged from 37.7 to 38.8 per cent. However, numerically higher oil content (38.8%) was recorded under T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₆ (30 kg S + 15 kg Zn ha⁻¹), followed by T₃ (0 kg S + 10 kg Zn ha⁻¹) and T₁₄ (30 kg S + 5 kg Zn ha⁻¹) with 38.6 per cent.

Although the lowest oil content (37.7%) was observed under control (T₁: 0 kg S + 0 kg Zn ha⁻¹), all treatments were found statistically at par, indicating non-significant effect of sulphur and zinc on oil percentage.

Data pertaining to oil yield showed significant variation among treatments. The maximum oil yield (939.2 kg ha⁻¹) was recorded under T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₆ (30 kg S + 15 kg Zn ha⁻¹), which were significantly superior over most of the treatments; however, these remained at par with T₃ (0 kg S + 10 kg Zn ha⁻¹) and T₁₄ (30 kg S + 5 kg Zn ha⁻¹). Although T₄ (0 kg S + 15 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹) also recorded higher oil yield (840.7 kg ha⁻¹), they were statistically at par with several treatments. The lowest oil yield (475.3 kg ha⁻¹) was recorded under control (T₁). Moreover, the increase in oil yield was mainly attributed to higher seed yield rather than oil content, as oil percentage did not vary significantly. Overall, data indicated that oil content was not significantly influenced by sulphur and zinc application and remained at par among treatments. However, oil yield was significantly affected and followed the trend of seed yield. Moreover, treatments T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₆ (30 kg S + 15 kg Zn ha⁻¹) proved superior in terms of oil yield, although these were statistically at par with T₃ and T₁₄. Nitrogen content (%) Data pertaining to nitrogen content in grain and straw as influenced by different levels of sulphur and zinc revealed significant variation among treatments. The maximum nitrogen content in grain (4.2%) was recorded under T₁₆ (30 kg S + 15 kg Zn ha⁻¹), which was significantly superior; however, it remained at par with T₃ (0 kg S + 10 kg Zn ha⁻¹), T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₂ (20 kg S + 15 kg Zn ha⁻¹). The lowest nitrogen content (3.1%) was recorded under control (T₁: 0 kg S + 0 kg Zn ha⁻¹). Similarly, nitrogen content in straw was found maximum (0.61%) under T₃ (0 kg S + 10 kg Zn ha⁻¹), although it remained at par with T₅ and T₁₆, whereas the minimum value (0.28%) was recorded under control (T₁). Moreover, the application of sulphur and zinc improved nitrogen concentration in plant tissues; however, several treatments were statistically at par, indicating marginal differences at higher levels.

Data pertaining to nitrogen uptake by grain and straw showed significant differences among treatments. The maximum nitrogen uptake by grain (99.2 kg ha⁻¹) was recorded under T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₆ (30 kg S + 15 kg Zn ha⁻¹), which were significantly superior over most of the treatments; however, these were found at par with T₃ (0 kg S + 10 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹). The lowest uptake (39.1 kg ha⁻¹) was recorded under control (T₁). Similarly, nitrogen uptake by straw was highest (65.1 kg ha⁻¹) under T₅ and T₁₆, whereas control recorded the lowest value (15.1 kg ha⁻¹). Moreover, increased nitrogen uptake with sulphur and zinc application may be attributed to higher biomass production and better nutrient absorption; however, several treatments remained statistically at par.

Data pertaining to phosphorus content in grain and straw as influenced by different levels of sulphur and zinc revealed significant variation among treatments. The maximum phosphorus content in grain (0.75%) was recorded under T₁₆ (30 kg S + 15 kg Zn ha⁻¹), which was significantly superior; however, it remained at par with T₃ (0 kg S + 10 kg Zn ha⁻¹), T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₄ (30 kg S + 5 kg Zn ha⁻¹). The lowest phosphorus content (0.46%) was recorded under control (T₁: 0 kg S + 0 kg Zn ha⁻¹). Similarly, phosphorus content in straw was found maximum (0.35%) under T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₆ (30 kg S + 15 kg Zn ha⁻¹), although these were at par with several treatments, whereas the minimum value (0.15%) was recorded under control (T₁). Moreover, the application of sulphur and zinc improved phosphorus concentration in plant tissues; however, several treatments remained statistically at par, indicating limited variation at higher nutrient levels.

Data pertaining to phosphorus uptake by grain and straw showed significant variation among treatments. The maximum phosphorus uptake by grain (18.2 kg ha⁻¹) was recorded under T₁₆ (30 kg S + 15 kg Zn ha⁻¹), which was significantly superior over most of the treatments; however, it remained at par with T₃ (0 kg S + 10 kg Zn ha⁻¹) and T₁₄ (30 kg S + 5 kg Zn ha⁻¹). The lowest uptake (5.8 kg ha⁻¹) was recorded under control (T₁). Similarly, phosphorus uptake by straw was highest (38.6 kg ha⁻¹) under T₁₆, followed by T₅ (10 kg S + 0 kg Zn ha⁻¹) with 37.6 kg ha⁻¹, although these were at par with several treatments. Moreover, increased phosphorus uptake under higher levels of sulphur and zinc may be attributed to enhanced root activity and better nutrient absorption; however, several treatments remained statistically at par.

Discussion

Growth parameters Plant height:

The results of the present investigation indicated that plant height increased significantly with increasing levels of sulphur and zinc. This may be attributed to the role of sulphur in synthesis of amino acids and proteins, which enhanced cell division and elongation. Zinc also plays an important role in auxin synthesis and enzymatic activity, thereby improving vegetative growth. The interaction effect of sulphur and zinc further enhanced plant height, indicating a synergistic effect between these nutrients. These findings are in close conformity with those reported by Kumar et al. (2018) [14] , Singh and Singh (2015) [51] and Jaiswal et al. (2025) [34] .

Number of branches:

The increase in number of branches with higher levels of sulphur and zinc might be due to better nutrient availability and enhanced metabolic activities. Sulphur helps in chlorophyll formation and improves photosynthesis, while zinc regulates growth hormones, leading to better branching. These results are in agreement with the findings of Sharma et al. (2014) [49] and Meena et al. (2016) [40] . Dry matter accumulation: Dry matter accumulation increased significantly due to sulphur and zinc application. This may be due to improved photosynthetic efficiency and nutrient uptake. Sulphur enhances protein synthesis, while zinc activates several enzymes, leading to increased biomass production. Similar findings were also reported by Ganvit et al. (2023) [33] and Kumar et al. (2021) [38] .

Yield attributes Number of siliqua per plant:

The increase in number of siliqua per plant with higher sulphur and zinc levels might be due to better flowering and reduced flower drop. Sulphur plays a key role in reproductive development, while zinc improves pollen viability. These results corroborate the findings of Bhinda et al. (2024) [8,28] and Choudhary et al.

Seeds per siliqua: The improvement in seeds per siliqua may be attributed to better fertilization and nutrient translocation. Zinc plays a vital role in seed formation, while sulphur contributes to protein synthesis. These findings are in accordance with Patel et al. (2015) [44] and Sultana et al. (2020) [23,54] . Test weight: Test weight increased with increasing levels of sulphur and zinc, which might be due to better seed filling and accumulation of photosynthates. Similar results were reported by Rathore et al. (2015) [45] and Singh et al. (2014) [52] .

Yield

Seed yield: Seed yield is the cumulative effect of growth and yield attributes. The significant increase in seed yield with sulphur and zinc application may be due to improved vegetative growth, higher number of siliqua and better seed development. The highest yield recorded under combined application of sulphur and zinc indicates a synergistic effect.

These findings are in agreement with Kumari et al. (2023) [39] ,

Bhambu et al. (2025) [7,27] and Jaiswal et al. (2025) [12,34] . Stover yield: Stover yield also increased due to enhanced vegetative growth and biomass accumulation under higher nutrient levels. Similar findings were reported by Nath et al.

(2019) [17,43] and Bhardwaj and Singh (2022) [29] .

Quality parameters

Oil content: Oil content increased significantly with sulphur and zinc levels. Sulphur is an essential component of certain amino acids and enzymes involved in oil synthesis. Zinc also influences metabolic activities related to oil formation. These findings are supported by Singh et al. (2024) [53] , Sharma et al. (2021) [50] and Aulakh et al. (2013) [25] . Economics: Higher net returns and benefit-cost ratio under higher levels of sulphur and zinc may be due to increased yield and better market value. Thus, balanced fertilization with sulphur and zinc proved economically beneficial. These results are in line with Nath et al. (2019) [17,43] and Kumari et al. (2023) [39] .

Conclusion

On the basis of the results obtained from the present investigation, it can be concluded that application of sulphur and zinc significantly influenced growth, yield, quality, nutrient uptake and economics of mustard crop.

International Journal of Agriculture and Food Science https://www.agriculturaljournals.com

Although higher levels of sulphur and zinc improved most of the parameters, the response was not always significantly different beyond optimum levels, as many treatments remained statistically at par. Treatment T₅ (10 kg S + 0 kg Zn ha⁻¹) and T₁₅ (30 kg S + 10 kg Zn ha⁻¹) proved superior in terms of seed yield and overall crop performance. However, treatment T₁₂ (20 kg S + 15 kg Zn ha⁻¹) and T₁₄ (30 kg S + 5 kg Zn ha⁻¹) were found most economical by recording higher net return and B:C ratio.

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