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Research article · 2025 · Full text

International Journal of Horticulture and Food Science

Documenting cold storage techniques of rice in the Malabar coast

Emre OzturkZeynep ArslanMurat Aksoy

Volume 7(5) · Pages 192–195 · DOI: 10.33545/26631067.2025.v7.i5c.631 ↗

Research areasRice Cultivation and Yield ImprovementPostharvest Quality and Shelf Life ManagementAgricultural Engineering and Mechanization

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Abstract

Author-written

Consider a grain warehouse on the Malabar Coast during monsoon: temperatures above 30 degrees Celsius, humidity touching 95%, and stored rice losing quality by the day. This research documented the performance of five cold storage techniques for paddy rice (Oryza sativa L.) under these challenging coastal conditions. The work was coordinated through the Izmir Institute of Agricultural Sciences between September 2021 and December 2023, evaluating hermetic sealing, controlled atmosphere storage, vacuum packing, desiccant-based storage, and conventional ambient storage over a 180-day monitoring period. Grain quality retention was highest under hermetic sealing (92.4%), followed by controlled atmosphere (88.7%) and vacuum packing (85.3%). Ambient storage retained only 63.1% of initial quality by day 180. Moisture content, insect infestation, mold growth, head rice yield, and cooking quality parameters were tracked monthly. Hermetic sealing maintained grain moisture within 0.3% of the initial value, while ambient storage showed a 2.8% moisture increase over the same period. A multi-criteria radar analysis revealed that no single technique excelled across all quality dimensions, with hermetic sealing scoring highest for moisture control and mold inhibition but lower for cost efficiency compared to vacuum packing. These results provide evidence-based recommendations for rice storage infrastructure investment in tropical coastal zones.

Summary

Source-checked

Plain-language reading of the full paper, prepared with AI and checked against the text. Each point links to the authors' own words.

  1. Why it matters

    • Rice storage in tropical coastal regions of South and Southeast Asia is inefficient, with losses ranging from 10% to 25% of the harvested crop. View evidence ↗

      Why it matters

      Rice storage in tropical coastal regions of South and Southeast Asia is inefficient, with losses ranging from 10% to 25% of the harvested crop.

      In the paper's own words

      “Rice storage losses in tropical coastal regions of South and Southeast Asia range from 10 to 25% of the harvested crop, translating to millions of tonnes of food wasted between the field and the consumer's plate”

      Page 1

      Found in: Introduction

      Read it in the full textOpen PDF at page 1 ↗
    • The Malabar Coast of southwestern India has high annual rainfall (2,500 to 3,500 mm) and average temperatures of 26 to 32 degrees Celsius, which accelerate the deterioration of stored grain. View evidence ↗

      Why it matters

      The Malabar Coast of southwestern India has high annual rainfall (2,500 to 3,500 mm) and average temperatures of 26 to 32 degrees Celsius, which accelerate the deterioration of stored grain.

      In the paper's own words

      “The Malabar Coast of southwestern India, stretching through Kerala and southern Karnataka, receives annual rainfall of 2,500 to 3,500 mm and maintains average temperatures of 26 to 32 degrees Celsius, creating an environment where grain deterioration accelerates without proper storage infrastructure”

      Page 1

      Found in: Introduction

      Read it in the full textOpen PDF at page 1 ↗
    • Aflatoxin contamination from Aspergillus species is a significant risk in these regions, making grain unfit for consumption if concentrations exceed 20 ppb. View evidence ↗

      Why it matters

      Aflatoxin contamination from Aspergillus species is a significant risk in these regions, making grain unfit for consumption if concentrations exceed 20 ppb.

      In the paper's own words

      “Aflatoxin contamination from Aspergillus species is a particular concern, as it renders entire grain lots unfit for consumption at concentrations above 20 ppb”

      Page 1

      Found in: Introduction

      Read it in the full textOpen PDF at page 1 ↗
  2. The question

    • The study aimed to compare five different storage techniques over 180 days under Malabar Coast conditions to determine which method best preserves rice quality. View evidence ↗

      The question

      The study aimed to compare five different storage techniques over 180 days under Malabar Coast conditions to determine which method best preserves rice quality.

      In the paper's own words

      “This research was designed to fill that gap by comparing five storage techniques over 180 days under Malabar Coast climatic conditions, measuring a comprehensive set of physical, chemical, and biological quality indicators to determine which approaches deliver the best overall grain preservation.”

      Page 2

      Found in: Introduction

      Read it in the full textOpen PDF at page 2 ↗
  3. What they did

    • The researchers used parboiled, medium-grain Jyothi rice, with initial moisture standardized to 13.8% wet basis. View evidence ↗

      What they did

      The researchers used parboiled, medium-grain Jyothi rice, with initial moisture standardized to 13.8% wet basis.

      In the paper's own words

      “Freshly milled rice of the variety Jyothi (medium-grain, parboiled) was obtained from the Kerala State Warehousing Corporation within two weeks of milling. Initial grain moisture was standardized to 13.8% wet basis using a fluidized-bed dryer.”

      Page 2

      Found in: Material

      Read it in the full textOpen PDF at page 2 ↗
    • The five tested treatments were hermetic bags, controlled atmosphere chambers (2% O2, 60% CO2), vacuum-sealed 5 kg bags, desiccant storage (5 g/kg silica gel), and ambient storage in gunny bags. View evidence ↗

      What they did

      The five tested treatments were hermetic bags, controlled atmosphere chambers (2% O2, 60% CO2), vacuum-sealed 5 kg bags, desiccant storage (5 g/kg silica gel), and ambient storage in gunny bags.

      In the paper's own words

      “Five storage treatments were established: hermetic bags (GrainPro SuperGrainBag, 1-tonne capacity), controlled atmosphere chambers (2% O2, 60% CO2, balance N2), vacuum-sealed 5 kg polyamide bags, desiccant storage (silica gel sachets at 5 g/kg grain in woven polypropylene bags), and ambient storage in standard gunny bags as the control”

      Page 2

      Found in: Material

      Read it in the full textOpen PDF at page 2 ↗
    • The trial took place at the Izmir Institute of Agricultural Sciences between September 2021 and December 2023, using 600 kg of grain per treatment across four replications. View evidence ↗

      What they did

      The trial took place at the Izmir Institute of Agricultural Sciences between September 2021 and December 2023, using 600 kg of grain per treatment across four replications.

      In the paper's own words

      “The trial was conducted at the Izmir Institute of Agricultural Sciences from September 2021 through December 2023... Each treatment used 600 kg of grain distributed across four replications.”

      Page 2

      Found in: Material

      Read it in the full textOpen PDF at page 2 ↗
    • Quality was measured monthly based on moisture, insect and mold counts, head rice yield, color, cooking quality, and aroma scores. View evidence ↗

      What they did

      Quality was measured monthly based on moisture, insect and mold counts, head rice yield, color, cooking quality, and aroma scores.

      In the paper's own words

      “Moisture content, insect count (live adults per kg), mold colony count (CFU/g), head rice yield (percentage of whole kernels after milling), grain color (L* a* b* values by Minolta CR-400), cooking quality (elongation ratio, water uptake), and aroma retention (sensory panel score on a 1-9 hedonic scale by eight trained panelists) were measured”

      Page 2

      Found in: Methods

      Read it in the full textOpen PDF at page 2 ↗
  4. What they found

    • By day 180, hermetic sealing had the highest quality retention at 92.4%, while ambient storage was the lowest at 63.1%. View evidence ↗

      What they found

      By day 180, hermetic sealing had the highest quality retention at 92.4%, while ambient storage was the lowest at 63.1%.

      In the paper's own words

      “Grain quality retention was highest under hermetic sealing (92.4%), followed by controlled atmosphere (88.7%) and vacuum packing (85.3%). Ambient storage retained only 63.1% of initial quality by day 180.”

      Page 1

      Found in: Methods

      Read it in the full textOpen PDF at page 1 ↗
    • Hermetic and controlled atmosphere storage kept moisture within 0.5% of initial levels, whereas ambient storage suffered severe insect infestation (18.3/kg) and mold growth (4.8 log CFU/g). View evidence ↗

      What they found

      Hermetic and controlled atmosphere storage kept moisture within 0.5% of initial levels, whereas ambient storage suffered severe insect infestation (18.3/kg) and mold growth (4.8 log CFU/g).

      In the paper's own words

      “At 180 days, hermetic and controlled atmosphere storage maintained grain moisture within 0.5% of the initial level (Table 2). ... Ambient storage showed severe insect infestation (18.3 per kg) and mold proliferation (4.8 log CFU/g)”

      Page 2

      Found in: Methods

      Read it in the full textOpen PDF at page 2 ↗
    • Both hermetic and controlled atmosphere storage completely suppressed insect populations. View evidence ↗

      What they found

      Both hermetic and controlled atmosphere storage completely suppressed insect populations.

      In the paper's own words

      “Insect populations were completely suppressed in both hermetic and controlled atmosphere treatments.”

      Page 2

      Found in: Methods

      Read it in the full textOpen PDF at page 2 ↗
    • Multi-criteria analysis showed hermetic sealing was best for moisture control (9.2/10) and mold inhibition (9.1/10), controlled atmosphere was best for color (8.9/10) and aroma (8.1/10), and vacuum packing was most cost-efficient (7.1/10). View evidence ↗

      What they found

      Multi-criteria analysis showed hermetic sealing was best for moisture control (9.2/10) and mold inhibition (9.1/10), controlled atmosphere was best for color (8.9/10) and aroma (8.1/10), and vacuum packing was most cost-efficient (7.1/10).

      In the paper's own words

      “The radar comparison (Figure 2) shows that hermetic sealing scored highest on moisture control (9.2/10) and mold inhibition (9.1/10), while controlled atmosphere led in color retention (8.9/10) and aroma preservation (8.1/10). Vacuum packing scored highest for cost efficiency (7.1/10).”

      Page 3

      Found in: Results

      Read it in the full textOpen PDF at page 3 ↗
    • Desiccant storage was less effective against extreme monsoon humidity and required silica gel replacement every 45-60 days. View evidence ↗

      What they found

      Desiccant storage was less effective against extreme monsoon humidity and required silica gel replacement every 45-60 days.

      In the paper's own words

      “The desiccant approach, while better than ambient, couldn't keep pace with the extreme humidity of the Malabar monsoon, and the silica gel sachets required replacement every 45-60 days to maintain effectiveness.”

      Page 4

      Found in: Introduction

      Read it in the full textOpen PDF at page 4 ↗
  5. What it means

    • The authors recommend using hermetic storage for bulk rice and vacuum packing for retail products in the Malabar Coast supply chain. View evidence ↗

      What it means

      The authors recommend using hermetic storage for bulk rice and vacuum packing for retail products in the Malabar Coast supply chain.

      In the paper's own words

      “The practical recommendation for the Malabar Coast rice supply chain is to adopt hermetic storage for bulk quantities, complemented by vacuum packing for consumer-facing products.”

      Page 4

      Found in: Introduction

      Read it in the full textOpen PDF at page 4 ↗
    • Future research is needed to evaluate the reusability of hermetic containers over multiple storage cycles and consecutive monsoon seasons. View evidence ↗

      What it means

      Future research is needed to evaluate the reusability of hermetic containers over multiple storage cycles and consecutive monsoon seasons.

      In the paper's own words

      “Further research should evaluate the long-term reusability of hermetic storage containers and their performance across multiple storage cycles spanning consecutive monsoon seasons.”

      Page 4

      Found in: Methods

      Read it in the full textOpen PDF at page 4 ↗

Visual summary

2 visuals

Illustrations explain the paper; charts are replotted from the paper's own table values.

AI-generated illustration
AI-generated conceptual illustration (rice grains in various storage containers). It explains the idea and does not show study data.↗ Enlarge
Comparison of Storage Mechanisms
AI-generated conceptual illustration (rice grains in various storage containers). It explains the idea and does not show study data.

AI-generated conceptual illustration (rice grains in various storage containers). It explains the idea and does not show study data.

Open full-size image ↗

Comparison of Storage Mechanisms

AI-generated conceptual illustration (rice grains in various storage containers). It explains the idea and does not show study data.

View source evidence ↗
Replotted from source data
Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.↗ Enlarge
Day 30 and Day 90 and more: Hermetic vs Controlled Atm. vs Vacuum Pack vs Desiccant vs Ambient
Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

Open full-size image ↗

Day 30 and Day 90 and more: Hermetic vs Controlled Atm. vs Vacuum Pack vs Desiccant vs Ambient

Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

View source evidence ↗

Figures & tables

From the original paper
Monthly quality retention index (%) under five storage techniquesTable 1Page 2Key quality parameters at day 180 across storage techniquesTable 2Page 2Grain quality retention under different cold storage techniques for riceFigure 1Page 3Multi-criteria performance comparison of top three storage methodsFigure 2Page 3
FULL TEXT Original article · reflowed for reading

Introduction

A single grain weevil can destroy 30% of a rice lot in under six months when storage conditions favor its reproduction, and the Malabar Coast offers precisely those conditions for most of the year [1] . Rice storage losses in tropical coastal regions of South and Southeast Asia range from 10 to 25% of the harvested crop, translating to millions of tonnes of food wasted between the field and the consumer's plate [2] . The Malabar Coast of southwestern India, stretching through Kerala and southern Karnataka, receives annual rainfall of 2,500 to 3,500 mm and maintains average temperatures of 26 to 32 degrees Celsius, creating an environment where grain deterioration accelerates without proper storage infrastructure [3] . Rice quality after harvest depends on maintaining low and stable moisture content, preventing insect and mold colonization, and limiting biochemical changes like lipid oxidation and starch retrogradation [4] . Conventional storage in gunny bags under ambient conditions fails on all three counts in tropical coastal settings. The high humidity drives moisture reabsorption into the grain, which in turn supports fungal growth and insect reproduction. Aflatoxin contamination from Aspergillus species is a particular concern, as it renders entire grain lots unfit for consumption at concentrations above 20 ppb [5] . Several improved storage technologies have been developed and tested in temperate and semi-arid zones. Hermetic storage, which creates an oxygen-depleted, carbon dioxideenriched atmosphere through the metabolic activity of grain and any insects present, has shown promising results in sub-Saharan Africa [6] . Controlled atmosphere storage allows precise manipulation of O 2 , CO 2 , and N 2 levels but requires more expensive infrastructure [7] . Vacuum packing removes air mechanically and is widely used for specialty rice products. Desiccant-based approaches use hygroscopic materials to absorb excess moisture from the grain mass [8] . Yet comparative data for these technologies under the specific conditions of the Malabar Coast, where the combination of heat and humidity is more extreme than most previously tested environments, are lacking. This research was designed to fill that gap by comparing five storage techniques over 180 days under Malabar Coast climatic conditions, measuring a comprehensive set of physical, chemical, and biological quality indicators to determine which approaches deliver the best overall grain preservation.

Instrumentation and analytical equipment

Grain moisture was measured with a Kett PM-450 nearinfrared moisture meter, calibrated against oven-dried samples (130 degrees Celsius for 24 hours, AOAC method 930.15). Insect counts used a Berlese funnel extraction system with a 40 W incandescent heat source. Mold enumeration followed the dilution plating technique on dichloran rose bengal chloramphenicol agar, incubated at 25 degrees Celsius for 5-7 days. Head rice yield was assessed using a Satake TM05C laboratory rice mill and a TR-260 length grader. Cooking quality measurements (elongation ratio, water uptake) used standardized IRRI protocols on a Labline water bath at 100 degrees Celsius [9] . Amylose content was determined colorimetrically with an iodinebinding method at 620 nm.

Quality assurance measures

All analytical instruments were calibrated monthly against certified reference materials. Moisture meter accuracy was verified within plus or minus 0.2% of the oven reference value. Temperature and humidity inside storage units were logged continuously with calibrated Tinytag Plus 2 data loggers at 30-minute intervals. Duplicate analyses were run for every tenth sample across all chemical parameters, with acceptable coefficient of variation set below 5%. Reference grain samples of known quality (IRRI GSR-001) were included in each analytical batch as external quality checks.

Material and Methods

Material and Methods

Material

Freshly milled rice of the variety Jyothi (medium-grain, parboiled) was obtained from the Kerala State Warehousing Corporation within two weeks of milling. Initial grain moisture was standardized to 13.8% wet basis using a fluidized-bed dryer. Five storage treatments were established: hermetic bags (GrainPro SuperGrainBag, 1tonne capacity), controlled atmosphere chambers (2% O 2 , 60% CO 2 , balance N2), vacuum-sealed 5 kg polyamide bags, desiccant storage (silica gel sachets at 5 g/kg grain in woven polypropylene bags), and ambient storage in standard gunny bags as the control [10] . The trial was conducted at the Izmir Institute of Agricultural Sciences from September 2021 through December 2023, with ethics clearance (Protocol IIAS-2021-028, approved August 18, 2021). Each treatment used 600 kg of grain distributed across four replications.

Methods

Grain samples (500 g) were drawn monthly from each replication for quality assessment. Moisture content, insect count (live adults per kg), mold colony count (CFU/g), head rice yield (percentage of whole kernels after milling), grain color (L* a* b* values by Minolta CR-400), cooking quality (elongation ratio, water uptake), and aroma retention (sensory panel score on a 1-9 hedonic scale by eight trained panelists) were measured [9] . A composite quality retention index was calculated by normalizing each parameter against its initial value and averaging across all seven parameters. Data were analyzed using repeated-measures ANOVA in SAS 9.4 with treatment as the main factor and sampling time as the repeated factor. Means were separated by Duncan's multiple range test at p<0.05 [11] . Radar charts were constructed from quality dimension scores normalized to a 0-10 scale. Quality retention declined progressively in all treatments over the 180-day period, but at significantly different rates (p<0.01; Table 1). Hermetic sealing maintained the slowest deterioration rate, losing only 6.3 percentage points from its initial value. Ambient storage lost 30.0 points over the same period. The sharpest quality drop in ambient storage occurred between days 60 and 120, coinciding with the peak monsoon humidity period. At 180 days, hermetic and controlled atmosphere storage maintained grain moisture within 0.5% of the initial level (Table 2). Insect populations were completely suppressed in both hermetic and controlled atmosphere treatments.

Table 2. Key quality parameters at day 180 across storage techniques
ParameterHermeticControlledVacuumDesiccantAmbient
Moisture (%wb)14.114.314.415.216.6
Insects (no./kg)0.00.00.24.718.3
Mold (log CFU/g)2.12.32.83.64.8
Head Rice (%)63.461.859.756.248.3
Aroma Score7.27.46.85.94.1

Table text extracted from the original PDF; superscript letters are shown inline. The original table image is below.

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Key quality parameters at day 180 across storage techniques↗ Enlarge
Table 2
Key quality parameters at day 180 across storage techniques

Key quality parameters at day 180 across storage techniques

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Original paper tableSource PDF · page 2 ↗
Table 1. Monthly quality retention index (%) under five storage techniques
TreatmentDay 30Day 90Day 120Day 150Day 180
Hermetic98.796.495.193.892.4
Controlled Atm.98.395.193.290.888.7
Vacuum Pack97.893.691.488.385.3
Desiccant96.490.887.283.178.6
Ambient93.182.476.369.763.1

Table text extracted from the original PDF; superscript letters are shown inline. The original table image is below.

View original table image
Monthly quality retention index (%) under five storage techniques↗ Enlarge
Table 1
Monthly quality retention index (%) under five storage techniques

Monthly quality retention index (%) under five storage techniques

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Original paper tableSource PDF · page 2 ↗

Results

Ambient storage showed severe insect infestation (18.3 per kg) and mold proliferation (4.8 log CFU/g), approaching the threshold for mycotoxin risk.

The bar chart (Figure 1) presents the final quality retention scores at day 180, highlighting the 29.3 percentage point gap between the best (hermetic, 92.4%) and worst (ambient, 63.1%) storage options. The radar comparison (Figure 2) shows that hermetic sealing scored highest on moisture control (9.2/10) and mold inhibition (9.1/10), while controlled atmosphere led in color retention (8.9/10) and aroma preservation (8.1/10).

Figure 2. Multi-criteria performance comparison of top three storage methods
Multi-criteria performance comparison of top three storage methods↗ Enlarge
Figure 2
Multi-criteria performance comparison of top three storage methods

Multi-criteria performance comparison of top three storage methods

Open full-size image ↗
Original paper figureSource PDF · page 3 ↗
Figure 1. Grain quality retention under different cold storage techniques for rice
Grain quality retention under different cold storage techniques for rice↗ Enlarge
Figure 1
Grain quality retention under different cold storage techniques for rice

Grain quality retention under different cold storage techniques for rice

Open full-size image ↗
Original paper figureSource PDF · page 3 ↗

Vacuum packing scored highest for cost efficiency (7.1/10).

Discussion

The strong performance of hermetic sealing in this research aligns with results from trials in East Africa and the Philippines, where similar technology reduced grain losses from 15-20% to below 3% [6] . The mechanism is straightforward: insect respiration within a sealed environment depletes oxygen and elevates carbon dioxide to lethal levels (typically below 2% O 2 and above 15% CO 2 )

within two to three weeks. This self-generating modified atmosphere also inhibits aerobic mold species, explaining the low fungal counts we observed at 2.1 log CFU/g after 180 days [12] . Controlled atmosphere storage achieved similar biological control but at considerably higher cost, requiring gas cylinders, regulators, and gas-tight chambers. Its advantage over hermetic sealing was marginal for insect and mold control, but it did deliver slightly better color and aroma retention. The constant atmosphere prevents the transient oxygen fluctuations that occur in hermetic bags as insect populations rise and fall, which may protect oxidationsensitive aroma compounds [7] . Whether the

Acknowledgement

Acknowledgements Funding sourcesThe Izmir Institute of Agricultural Sciences provided internal research funding for this work.

References

18 in the original paper
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  12. Villers P, Navarro S, De Bruin T. Development of hermetic storage technology in sealed bags for grain storage. CAB Reviews. 2010;5(12):1-10.
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  16. Boxall RA. Post-harvest losses to insects: a world overview. International Biodeterioration and Biodegradation. 2001;48(1-4):137-152.
  17. Jayas DS, White NDG. Storage and drying of grain in Canada. Journal of Stored Products Research. 2003;39(3):219-228.
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