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Research article · 2024 · Full text

International Journal of Horticulture and Food Science

Optimization of soilless culture systems for year-round production of lettuce in vertical farms

R Chithiraichelvan

Volume 6(1) · Pages 74–78 · DOI: 10.33545/26631067.2024.v6.i1a.471 ↗

Research areasInnovations in Aquaponics and Hydroponics SystemsLight effects on plantsGreenhouse Technology and Climate Control

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Abstract

Author-written

Vertical farming represents a paradigm shift in agricultural production, enabling cultivation of leafy vegetables in controlled environments independent of external climate constraints. This research compared the performance of five soilless culture systems for lettuce (Lactuca sativa L.) production in a multi-tier vertical farm facility equipped with LED lighting and climate control. Systems evaluated included nutrient film technique (NFT), deep water culture (DWC), aeroponics, ebb-and-flow, and drip irrigation on substrate. The experiment followed completely randomized design with five replications per system, testing butter head lettuce variety across four consecutive production cycles. Parameters measured encompassed yield per unit area, water use efficiency, energy consumption, growth rate, and quality attributes including fresh weight, leaf number, chlorophyll content, and nitrate accumulation. Results demonstrated that aeroponics achieved maximum yield of 22.6 kg/m² per cycle, significantly exceeding NFT at 18.4 kg/m² and DWC at 16.2 kg/m². Water use efficiency in aeroponics reached 8.4 L/kg compared to 12.6 L/kg in NFT and 18.6 L/kg in drip systems. However, energy consumption for aeroponics at 12.4 kWh/kg was highest among systems due to continuous misting requirements. NFT provided optimal balance between yield, resource efficiency, and operational simplicity, making it suitable for commercial adoption. Lettuce quality showed minimal variation among hydroponic systems but nitrate levels were significantly lower in aeroponic production. Growth curves indicated faster development in aeroponics with harvest maturity achieved in 28 days compared to 35 days in substrate-based drip systems. Year-round production feasibility was confirmed across all systems with consistent yields regardless of external seasonal conditions. Economic analysis factoring capital costs, operating expenses, and yield returns identified NFT as most profitable for commercial vertical farm operations.

Summary

Source-checked

Plain-language reading of the full paper, prepared with AI and checked against the text. Each point links to the authors' own words.

  1. Why it matters

    • Global food demand is expected to rise by 70% by the middle of the century due to population growth and dietary changes. View evidence ↗

      Why it matters

      Global food demand is expected to rise by 70% by the middle of the century due to population growth and dietary changes.

      In the paper's own words

      “Analysts project that global food demand will increase by 70% before mid-century, driven by population growth and changing dietary preferences in developing economies.”

      Page 1

      Found in: Introduction

      Read it in the full textOpen PDF at page 1 ↗
    • Traditional farming is increasingly limited by the loss of farmland to cities, water shortages, and unpredictable climate patterns. View evidence ↗

      Why it matters

      Traditional farming is increasingly limited by the loss of farmland to cities, water shortages, and unpredictable climate patterns.

      In the paper's own words

      “Conventional agricultural systems face mounting constraints from urbanization consuming productive farmland, water scarcity affecting irrigation availability, and climate variability disrupting traditional growing seasons”

      Page 1

      Found in: Introduction

      Read it in the full textOpen PDF at page 1 ↗
    • Vertical farming uses stacked layers and LED lighting to increase production per unit of land area. View evidence ↗

      Why it matters

      Vertical farming uses stacked layers and LED lighting to increase production per unit of land area.

      In the paper's own words

      “Vertical farming maximizes production per unit land area by stacking growing layers within enclosed structures, typically utilizing LED lighting to provide photosynthetically active radiation tailored to crop requirements”

      Page 1

      Found in: Introduction

      Read it in the full textOpen PDF at page 1 ↗
  2. The question

    • The study aimed to compare five different soilless culture systems to find the best configuration for commercial lettuce production in vertical farms. View evidence ↗

      The question

      The study aimed to compare five different soilless culture systems to find the best configuration for commercial lettuce production in vertical farms.

      In the paper's own words

      “This research therefore compared five soilless culture systems for lettuce production in a climate-controlled vertical farm to identify optimal configurations for commercial adoption”

      Page 2

      Found in: Introduction

      Read it in the full textOpen PDF at page 2 ↗
  3. What they did

    • The study used a four-tier vertical farm with LED lighting (250 µmol/m²/s, 16-hour photoperiod), temperature at 22±2°C, and humidity at 65±5%. View evidence ↗

      What they did

      The study used a four-tier vertical farm with LED lighting (250 µmol/m²/s, 16-hour photoperiod), temperature at 22±2°C, and humidity at 65±5%.

      In the paper's own words

      “The experiment was conducted in a purpose-built vertical farm facility comprising four cultivation tiers with LED lighting providing 250 µmol/m²/s photosynthetically active radiation at 16-hour photoperiod. Climate control maintained temperature at 22±2°C and relative humidity at 65±5% throughout the experimental period”

      Page 2

      Found in: Material

      Read it in the full textOpen PDF at page 2 ↗
    • Five systems were tested over four cycles with five replications each: Nutrient Film Technique (NFT), Deep Water Culture (DWC), Aeroponics, Ebb and Flow, and Drip irrigation on perlite. View evidence ↗

      What they did

      Five systems were tested over four cycles with five replications each: Nutrient Film Technique (NFT), Deep Water Culture (DWC), Aeroponics, Ebb and Flow, and Drip irrigation on perlite.

      In the paper's own words

      “Five soilless culture systems were evaluated in completely randomized design with five replications per system across four consecutive production cycles. Systems included S1-Nutrient Film Technique using 10 cm wide channels with 2 mm solution depth, S2-Deep Water Culture with 20 cm solution depth and continuous aeration, S3-Aeroponics with high-pressure misting at 60-second intervals, S4-Ebb and Flow with 15-minute flood cycles every 4 hours, and S5-Drip irrigation on perlite substrate with 6 irrigations daily”

      Page 2

      Found in: Material

      Read it in the full textOpen PDF at page 2 ↗
    • All systems used a standard Hoagland nutrient solution with a pH of 6.0 and an electrical conductivity (EC) of 1.8 dS/m. View evidence ↗

      What they did

      All systems used a standard Hoagland nutrient solution with a pH of 6.0 and an electrical conductivity (EC) of 1.8 dS/m.

      In the paper's own words

      “Standard Hoagland nutrient solution was maintained at EC 1.8 dS/m and pH 6.0 across all systems.”

      Page 2

      Found in: Material

      Read it in the full textOpen PDF at page 2 ↗
  4. What they found

    • Aeroponics produced the highest yield at 22.6 kg/m² per cycle, which was 22.8% higher than NFT and 82.3% higher than drip systems. View evidence ↗

      What they found

      Aeroponics produced the highest yield at 22.6 kg/m² per cycle, which was 22.8% higher than NFT and 82.3% higher than drip systems.

      In the paper's own words

      “Aeroponics achieved maximum productivity of 22.6 kg/m² per cycle, representing 22.8% improvement over NFT and 82.3% over substrate-based drip systems.”

      Page 2

      Found in: Discussion:

      Read it in the full textOpen PDF at page 2 ↗
    • Aeroponics was the most water-efficient system at 8.4 L/kg, followed by NFT at 12.6 L/kg, while substrate-based systems used the most water. View evidence ↗

      What they found

      Aeroponics was the most water-efficient system at 8.4 L/kg, followed by NFT at 12.6 L/kg, while substrate-based systems used the most water.

      In the paper's own words

      “Water use efficiency showed inverse relationship with system complexity, with aeroponics achieving best efficiency at 8.4 L/kg produced. ... NFT demonstrated acceptable water use at 12.6 L/kg, while substrate-based systems exhibited highest consumption”

      Page 2

      Found in: Material

      Read it in the full textOpen PDF at page 2 ↗
    • All tested systems were capable of year-round production with consistent yields, regardless of the season. View evidence ↗

      What they found

      All tested systems were capable of year-round production with consistent yields, regardless of the season.

      In the paper's own words

      “Year-round production feasibility was confirmed across all systems with consistent yields regardless of external seasonal conditions.”

      Page 1

      Found in: Discussion:

      Read it in the full textOpen PDF at page 1 ↗
  5. What it means

    • The authors conclude that the Nutrient Film Technique (NFT) is the most suitable for commercial use because it balances yield, efficiency, and simplicity. View evidence ↗

      What it means

      The authors conclude that the Nutrient Film Technique (NFT) is the most suitable for commercial use because it balances yield, efficiency, and simplicity.

      In the paper's own words

      “NFT provided optimal balance between yield, resource efficiency, and operational simplicity, making it suitable for commercial adoption.”

      Page 1

      Found in: Discussion:

      Read it in the full textOpen PDF at page 1 ↗
    • Economic analysis identified NFT as the most profitable system for commercial vertical farming. View evidence ↗

      What it means

      Economic analysis identified NFT as the most profitable system for commercial vertical farming.

      In the paper's own words

      “Economic analysis factoring capital costs, operating expenses, and yield returns identified NFT as most profitable for commercial vertical farm operations.”

      Page 1

      Found in: Discussion:

      Read it in the full textOpen PDF at page 1 ↗
    • The study is limited by the use of only one lettuce variety, which may not reflect the performance of other cultivars or species. View evidence ↗

      What it means

      The study is limited by the use of only one lettuce variety, which may not reflect the performance of other cultivars or species.

      In the paper's own words

      “This research tested a single lettuce variety that may not represent performance of other cultivars or leafy vegetable species.”

      Page 4

      Found in: Discussion:

      Read it in the full textOpen PDF at page 4 ↗
    • The experimental period was not long enough to fully capture long-term maintenance needs and system reliability. View evidence ↗

      What it means

      The experimental period was not long enough to fully capture long-term maintenance needs and system reliability.

      In the paper's own words

      “Long-term system reliability and maintenance requirements were not fully captured within the experimental period.”

      Page 4

      Found in: Discussion:

      Read it in the full textOpen PDF at page 4 ↗

Visual summary

3 visuals

Illustrations explain the paper; charts are replotted from the paper's own table values.

AI-generated illustration
AI-generated conceptual illustration (lettuce plants in vertical farm hydroponic systems). It explains the idea and does not show study data.↗ Enlarge
Resource vs. Productivity Trade-off
AI-generated conceptual illustration (lettuce plants in vertical farm hydroponic systems). It explains the idea and does not show study data.

AI-generated conceptual illustration (lettuce plants in vertical farm hydroponic systems). It explains the idea and does not show study data.

Open full-size image ↗

Resource vs. Productivity Trade-off

AI-generated conceptual illustration (lettuce plants in vertical farm hydroponic systems). It explains the idea and does not show study data.

View source evidence ↗
Replotted from source data
Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.↗ Enlarge
Yield (kg/m²): NFT vs DWC vs Aeroponics vs Ebb-Flow vs Drip
Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

Open full-size image ↗

Yield (kg/m²): NFT vs DWC vs Aeroponics vs Ebb-Flow vs Drip

Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

View source evidence ↗
Replotted from source data
Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.↗ Enlarge
Water Use (L/kg): NFT vs DWC vs Aeroponics vs Ebb-Flow vs Drip
Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

Open full-size image ↗

Water Use (L/kg): NFT vs DWC vs Aeroponics vs Ebb-Flow vs Drip

Replotted from Table 1 (page 2) of the original paper. Every bar is the paper's own table value.

View source evidence ↗

Figures & tables

From the original paper
Performance comparison of soilless culture systems for lettuce productionTable 1Page 2Lettuce quality parameters under different soilless culture systemsTable 2Page 3Heat map visualization of soilless culture system performance across multiple parametersFigure 1Page 3Comparative yield performance of different soilless culture systems for lettuceFigure 2Page 3Growth progression curves comparing NFT and aeroponic systems for lettuce productionFigure 3Page 4Schematic representation of multi-tier vertical farm structure with LED lightingFigure 4Page 4
FULL TEXT Original article · reflowed for reading

Introduction

Analysts project that global food demand will increase by 70% before mid-century, driven by population growth and changing dietary preferences in developing economies. Conventional agricultural systems face mounting constraints from urbanization consuming productive farmland, water scarcity affecting irrigation availability, and climate variability disrupting traditional growing seasons [1] . These converging pressures have stimulated innovation in controlled environment agriculture where production occurs independent of external conditions, enabling consistent supply of fresh produce regardless of geographic location or seasonal limitations [2] . Vertical farming maximizes production per unit land area by stacking growing layers within enclosed structures, typically utilizing LED lighting to provide photosynthetically active radiation tailored to crop requirements [3] . This approach can achieve productivity levels 20 to 100 times greater than field production on equivalent footprint areas while reducing water consumption by 90% or more compared to conventional irrigation agriculture [4] . The controlled environment eliminates pesticide requirements, minimizes food safety risks, and enables production within urban centers proximate to consumer markets. Leafy vegetables particularly lettuce have emerged as preferred crops for vertical farming owing to their rapid growth cycles, high value per unit weight, and consumer demand for fresh locally produced greens [5] . Lettuce adapts well to hydroponic cultivation and tolerates the relatively low light intensities economically feasible with current LED technology. The short production cycle of 30-45 days enables multiple harvests annually from the same growing positions [6] . Soilless culture systems differ fundamentally in how nutrient solution contacts plant roots, influencing oxygen availability, disease risk, and resource efficiency [7] . The nutrient film technique maintains a shallow flow of solution over roots confined in channels, providing continuous nutrient access with adequate aeration. Deep water culture suspends roots directly in aerated nutrient solution, while aeroponics delivers nutrients as fine mist to roots suspended in air [8] . Each system presents distinct advantages and limitations regarding capital requirements, operational complexity, and crop performance. Previous comparisons of hydroponic systems have yielded variable results depending on crops tested, environmental conditions, and management protocols employed [9] . Systematic evaluation under standardized vertical farm conditions would provide evidence-based guidance for commercial operations selecting production systems appropriate to their circumstances and objectives. This research therefore compared five soilless culture systems for lettuce production in a climate-controlled vertical farm to identify optimal configurations for commercial adoption [10] .

Material and Methods

Material and Methods

Material

The experiment was conducted in a purpose-built vertical farm facility comprising four cultivation tiers with LED lighting providing 250 µmol/m²/s photosynthetically active radiation at 16-hour photoperiod. Climate control maintained temperature at 22±2°C and relative humidity at 65±5% throughout the experimental period [11] . Butterhead lettuce variety Rex was selected for its commercial popularity and adaptation to hydroponic systems. Seeds were germinated in rockwool cubes and transplanted to treatment systems at the two true leaf stage.

Methods: Five soilless culture systems were evaluated in completely randomized design with five replications per system across four consecutive production cycles. Systems included S1-Nutrient Film Technique using 10 cm wide channels with 2 mm solution depth, S2-Deep Water Culture with 20 cm solution depth and continuous aeration, S3-Aeroponics with high-pressure misting at 60-second intervals, S4-Ebb and Flow with 15-minute flood cycles every 4 hours, and S5-Drip irrigation on perlite substrate with 6 irrigations daily [12] . Standard Hoagland nutrient solution was maintained at EC 1.8 dS/m and pH 6.0 across all systems. Yield was determined as fresh weight per square meter growing area at harvest maturity. Water use was monitored through flow meters recording total consumption per system. Energy consumption included lighting, climate control, and system-specific requirements measured through dedicated meters. Quality parameters comprised head weight, leaf number, chlorophyll content by SPAD meter, and nitrate concentration by ion-selective electrode [13] . Data were analyzed using analysis of variance with treatment separation by Tukey's test at 5% probability level.

Resource Utilization: Water consumption varied substantially among systems reflecting differences in evaporative losses and drainage requirements. Aeroponic systems achieved lowest water use through efficient mist delivery directly to roots with minimal runoff. NFT showed intermediate efficiency with recirculating solution minimizing waste, while substrate-based drip systems exhibited highest consumption due to drainage losses and evaporation from media surfaces [14] . Energy partitioning analysis revealed that lighting accounted for 65-75% of total consumption across systems, with system-specific equipment contributing the remainder.

Results

Results

Discussion:

The superior yield performance of aeroponics reflects fundamental advantages of root-zone oxygen availability that liquid-based systems cannot fully replicate [7] . Roots suspended in air with periodic misting maintain aerobic respiration supporting rapid nutrient uptake and growth. The enhanced oxygenation also reduces pathogen pressure from water-borne diseases that can accumulate in recirculating liquid systems. Lower nitrate accumulation in aeroponic lettuce carries significance for food safety given regulatory limits on nitrate content in leafy vegetables in many markets [8] . The improved nitrogen metabolism under aerobic root conditions apparently favors assimilation over accumulation, producing healthier product. This quality advantage may justify premium pricing that offsets higher production costs. NFT emerged as the practical optimum balancing yield, efficiency, and operational simplicity. The passive flow design minimizes equipment requirements and failure points compared to pressurized aeroponic systems [9] . Commercial operators prioritizing reliability over maximum productivity may prefer NFT for its proven track record and lower technical complexity. Energy consumption patterns highlight the trade-off between productivity and resource efficiency inherent in system selection. While aeroponics achieved highest yields, its continuous misting requirements elevated energy costs substantially [10] . Operations in regions with high electricity costs should factor this into economic projections when comparing system options.

Limitations: This research tested a single lettuce variety that may not represent performance of other cultivars or leafy vegetable species. The controlled environment conditions may not reflect challenges encountered in commercial facilities with less precise climate control. Long-term system reliability and maintenance requirements were not fully captured within the experimental period. Energy costs vary substantially among regions affecting economic conclusions. NFT provided optimal balance between productivity and operational practicality for commercial adoption. All systems supported year-round production with consistent yields across seasonal cycles, confirming the climate-independence advantage of vertical farming. Growth cycles ranged from 28 days in aeroponics to 38 days in substrate systems, enabling 9-13 harvest cycles annually depending on system selection. Quality parameters including chlorophyll content and nitrate levels favored aeroponic production, suggesting premium market positioning potential that may offset higher production costs. Operators should select systems aligned with their specific objectives regarding productivity versus efficiency and capital versus operating cost preferences. These findings provide quantitative benchmarks for vertical farm planning and optimization, supporting evidence-based decisions on production system investments.

Conclusion:

Conclusion:

Figures and tables from the paper

Table 1. Performance comparison of soilless culture systems for lettuce production
SystemYield (kg/m²)Water Use (L/kg)Energy (kWh/kg)Days to Harvest
NFT18.412.68.432
DWC16.214.86.834
Aeroponics22.68.412.428
Ebb-Flow14.816.27.236
Drip12.418.65.838
SEm(±)0.860.640.421.2
CD (p=0.05)2.722.021.333.8

Table text extracted from the original PDF; superscript letters are shown inline. The original table image is below.

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Performance comparison of soilless culture systems for lettuce production↗ Enlarge
Table 1
Performance comparison of soilless culture systems for lettuce production

Performance comparison of soilless culture systems for lettuce production

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Original paper tableSource PDF · page 2 ↗
Table 2. Lettuce quality parameters under different soilless culture systems
SystemHead Wt (g)Leaf NumberSPAD ValueNitrate (mg/kg)
NFT286.424.638.41842
DWC268.222.836.82124
Aeroponics342.828.442.61426
Ebb-Flow248.621.235.42286
Drip224.419.634.22468
SEm(±)12.41.181.2486
CD (p=0.05)39.23.723.92272

Table text extracted from the original PDF; superscript letters are shown inline. The original table image is below.

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Lettuce quality parameters under different soilless culture systems↗ Enlarge
Table 2
Lettuce quality parameters under different soilless culture systems

Lettuce quality parameters under different soilless culture systems

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Original paper tableSource PDF · page 3 ↗
Figure 1. Heat map visualization of soilless culture system performance across multiple parameters
Heat map visualization of soilless culture system performance across multiple parameters↗ Enlarge
Figure 1
Heat map visualization of soilless culture system performance across multiple parameters

Heat map visualization of soilless culture system performance across multiple parameters

Open full-size image ↗
Original paper figureSource PDF · page 3 ↗
Figure 2. Comparative yield performance of different soilless culture systems for lettuce
Comparative yield performance of different soilless culture systems for lettuce↗ Enlarge
Figure 2
Comparative yield performance of different soilless culture systems for lettuce

Comparative yield performance of different soilless culture systems for lettuce

Open full-size image ↗
Original paper figureSource PDF · page 3 ↗
Figure 3. Growth progression curves comparing NFT and aeroponic systems for lettuce production
Growth progression curves comparing NFT and aeroponic systems for lettuce production↗ Enlarge
Figure 3
Growth progression curves comparing NFT and aeroponic systems for lettuce production

Growth progression curves comparing NFT and aeroponic systems for lettuce production

Open full-size image ↗
Original paper figureSource PDF · page 4 ↗
Figure 4. Schematic representation of multi-tier vertical farm structure with LED lighting
Schematic representation of multi-tier vertical farm structure with LED lighting↗ Enlarge
Figure 4
Schematic representation of multi-tier vertical farm structure with LED lighting

Schematic representation of multi-tier vertical farm structure with LED lighting

Open full-size image ↗
Original paper figureSource PDF · page 4 ↗

References

17 in the original paper
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  16. Jones JB Jr. Hydroponics: a practical guide for the soilless grower. 2nd ed. Boca Raton (FL): CRC Press; 2005.
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