Phytochemical investigation and HPTLC of Cycas revoluta-based topical ointment

Abstract

Cycas revoluta, commonly known as the Sago palm, is an ornamental plant native to Southeast Asia and is classified as a gymnosperm under the Cycadaceae family. Historically, it has been utilized by various tribal communities for diverse medicinal and culinary applications. Distinct parts of the Sago palm are employed in treating numerous ailments, including estrogen-dependent carcinoma, different forms of cancer, liver tumors, diarrhea, dysentery, flatulence, vomiting, hemorrhoids, and wounds. The current research focuses on preliminary phytochemical screening, fluorescence analysis, and the interaction of chemical reagents with various phytoconstituents found in different extracts of the plant. Additionally, High-Performance Thin-Layer Chromatography (HPTLC) studies have been conducted using ethanolic extracts from the leaves to further explore its components. The preparation of the ointment base involved using a levigation method to integrate the herbal extract into the base, resulting in the formulation of a herbal ointment. Upon formulation, various physicochemical parameters were assessed to ensure its quality, including color, odor, pH level, spreadability, extrudability, consistency, solubility, and washability, providing an understanding of the ointment's characteristics and performance.