Study of the effects of dietary administration of Probiotic (Bacillus subtilis) on the haematological parameters of the fish ‘Labeo rohita’

Abstract

The effects of feed rich in probiotic Bacillus subtilis on the haematological parameters of the fish Labeo rohita were investigated in this study. Depending on the needs of the experiment, different combinations of the probiotic Bacillus subtilis were added to the basal feed. For ninety days, feeding studies were carried out in triplicate. Fishes of approximately similar body weights were randomly distributed in five glass tanks that were continually aired at the Department of Zoology, Bareilly College, Bareilly, Uttar Pradesh, for this experiment. Five concentrations of probiotic Bacillus subtilis (0%, 0.5%, 1%, 1.5%, and 2%) were administered concurrently to these five fish treatment groups. Treatment group T1, which served as the control group, was given a basal meal consisting 0% probiotic Bacillus subtilis. After a 15-day interval (viz 0, 15, 30, 45, 60, 75 and 90 days), haematological parameters (RBC count, Haemoglobin, Haematocrit value, Mean cell haemoglobin concentration, Mean cell haemoglobin, Mean cell volume) were measured to assess the effects of the probiotic Bacillus subtilis. Compared to other treatment groups, two treatment groups (T4 and T3) shown superior haematological parameters. In order to improve the effectiveness of fish feed, 1.5% probiotic Bacillus subtilis concentration in the basal diet of Labeo rohita is effective in aquaculture practices.