High expression of recombinant bacteria (Sak) and activity study’s author

Abstract

Previous research and studies have shown the usefulness of using Staphylokinase as a thrombolytic treatments reported to have a therapeutic function and used as a potential alternative for the available clot dissolving drugs and some countries proved to be a potent alternative over the available clot dissolving drugs. The paper detailed the use of the Escherichia coli strain BL12DE3 for protein synthesis using the pET21a vector, and BL12DE3 activates the T7 polymerase, which encodes LacI, using the pET21a vector. It has been established that the highest level of protein expression may be obtained in the shortest amount of time. SDS-PAGE was used to obtain a more than 44% soluble protein, which was then purified using chromatography. The mature recombinant sak protein expressed in E.coli is also further extracted and analyzed by various methods to demonstrate thrombolytic activity.