Influence of different polybag size on budding success of Nagpur mandarin
Abstract
An experiment entitled “Influence of different polybag size on budding success in Nagpur Mandarin” was carried out at Nursery Unit, Dr. Panjabrao Deshmukh Krishi Vidhyapeeth, Akola during the year 2023-24. The study aimed to standardized the ideal polybag size for the commercial propagation of Nagpur mandarin. The experiment was laid out in Factorial Completely Randomized Design consisted of different budding techniques as Factor A viz., B1 (Shield Budding), B2 (Chip Budding) and B3 (Softwood Grafting) and Polybag size as Factor B viz., P1 (5ʺx12ʺ), P2 (6ʺx12ʺ), P3 (7ʺx12ʺ) and P4 (8ʺx12ʺ) with twelve treatment combinations replicated three times. In respect of polybag size observations recorded before budding plant height (cm), number of leaves and leaf area (cm2), fresh weight of roots (g), dry weight of roots (g), fresh weight of shoots (g), dry weight of shoots (g), root to shoot ratio of fresh weight and root to shoot ratio of dry weight after 180 days to budding. Regarding the various polybag sizes, data collected 180 days after transplanting indicated that the 7ʺx12ʺ polybag produced better results in terms of rootstock characteristics, plant height (cm), leaf count, and leaf area (cm2). Fresh weight of roots (g), dry weight of roots (g), fresh weight of shoots (g), dry weight of shoots (g), root to shoot ratio of fresh weight and root to shoot ratio of dry weight found in P4 (7ʺx12ʺ polybag) after 180 days to budding.
Full Text
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Introduction
Citrus is the collective generic term comprising a number of species and varieties of fruits, known for their characteristic flavour and attractive range of colours. (T.K. Chattopadhyay, 1998). Citrus fruits have a prominent place among popular and extensively grown tropical and subtropical fruits. The citrus fruit, or hesperidium, is a unique berry with an edible pulp called endocarp that surrounds the juicy segment with non-endospermic seeds. The outermost layer, flavedo (zest), contains essential oil used in culinary purposes. The mesocarp, also known as the albedo, is the inner, colourless part of the fruit. The vitamin C content varies from 30 mg in C. aurantium to 68 mg in C. reticulata. The genus includes 16 species, as mentioned by Swingle in 1948 and 144 species by Tanaka in 1954 (Bose et al., 2001) [1] . Among citrus group major crops like oranges, mandarins, lemons, grapefruits, pummelos, and limes are grown commercially in all over India. Among all citrus fruits, mandarins are the most important one grown in India. Nagpur mandarin is leading fruit among citrus with production volume estimated at over 6 million metric tons according to reports of National Horticulture Board (NHB). Nagpur mandarin being a demanding fruit for both local consumption and export, it is necessary to increase production of healthy, disease free and early planting material. For economic production there is need to standardized polybag size for commercial production of Nagpur mandarin. The success of propagation method, however, depends on different factors like environmental conditions, polybag size, propagation technique and nutrient application. Accordingly, in the light of this premise, the present research work was carried out to figure out the influence of different budding techniques on budding success of Nagpur mandarin.
Materials and Methods
The experiment entitled "Influence of different budding techniques on budding success of Nagpur mandarin" was carried out at Fruit Nursery, Department of Fruit Science, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra during the year 2023-24 with the objectives to study the effect of different polybag size on budding success of Nagpur mandarin. The experiment was laid out in Factorial Completely Randomized Design with Factor A as different budding method viz., B 1 (Shield budding), B 2 (Chip budding) and B 3 (Softwood grafting) and Factor B as different polybag size viz., P 1 (5ʺx12ʺ), P 2 (6ʺx12ʺ), P 3 (7ʺx12ʺ) and P 4 (8ʺx12ʺ) with twelve treatment combinations replicated three times.
Methodology
Four polybag size of 5x12, 6x12, 7x12, 8x12 were used for raising the Rangpur lime rootstock. Potting mixture used is soil, sand and FYM in ratio 2:1:1. These rootstocks are used for budding after 180 days of transplanting. After budding observations were recorded after 180 days.
Plant Height
Scion length was recorded after 180 days of success grafting for five plants from each replication of all treatments by measuring scale.
Number of leaves
Number of leaves on scion was recorded after 180 days of budding and after computing the mean it was recorded as number of leaves.
Five plants from each replication in each treatment were collected and fresh weight of each root was recorded. The mean fresh weight of roots was calculated from the total number of fresh weights of all roots from each replication and expressed in grams (g).
Five plants from each replication in each treatment which were collected for fresh weight are used for dry weight. These roots of plant are kept under atmospheric temperature for three days then in hot air oven at 60-degree centigrade temperature. Mean of all five plants are taken and dry weight of roots was recorded.
Observations recorded: Data was collected after 180 days of transplanting to study the effect of polybag size on budding success of Nagpur mandarin. The observations are plant height (cm), leaf count, leaf area (cm2) recorded 180 days after transplanting and observations fresh weight of roots (g), dry weight of roots (g), fresh weight of shoots (g), dry weight of shoots (g), shoot to root ratio of fresh weight and shoot to root ratio of dry weight.
Design and statistical analysis: The experiment followed a Factorial Completely Randomized Design, with twelve treatment combinations, each replicated three times. A total of 30 plants were used per replication, following the methodology outlined by Panse and Sukhatme (1997) [6] .
Results and Discussion
The polybag size 7x12 is significantly superior in plant height (cm), number of leaves and leaf area (cm 2 ) after 180 days of transplanting.
Height is one of the primary factor influence by container size. The data presented in Table 1 shows significant differences in plant height of rootstock Rangpur lime after 180 days of transplanting. Significantly maximum (55.44 cm) plant height of rootstock Rangpur lime was observed in P 3 (polybag 7ʺx12ʺ) which was followed by (51.89 cm) in P 4 (polybag 8x12). The results are in conformity with Nesmith & Duval, 1992.
Number of leaves
The data presented in Table 1 represents significant differences in number of leaves of rootstock Rangpur lime after 180 days of transplanting. Significantly maximum (57.40) leaves were observed in P 3 (polybag 7ʺx12ʺ) which was followed by (54.96) in P 4 (polybag 8ʺx12ʺ). Number of leaves is influence by nutrition transfer to branches. 7ʺx12ʺ polybag provide enough volume for nutrient retention and absorption.
Significantly maximum (28.11cm 2 ) leaf area was observed in P 3 (polybag 7ʺx12ʺ) which was followed by (27.26 cm 2 ) in P 4 (polybag 8ʺx12ʺ). Leaf area is of significance in terms of photosynthesis competence as well as relativity to plant yield. Comparing the yield of plants in 5ʺx12ʺ and 6ʺx12ʺ polybags, the reduction of the root area results in less nutrient and water absorption, giving small leaves and a small leaf area (Marler & Davies, 1987) [4] .
After 180 days of budding, the data in Table 2 shows noteable variations in the fresh weight of roots of rootstock Rangpur lime. Significantly maximum (16.54 g) weight was observed in P 3 (polybag 7ʺx12ʺ) which was followed by (16.08 g) in P 4 (polybag 8ʺx12ʺ). Growth and development of roots directly influence the fresh weight of roots. Medium polybag size gives optimum space for roots development which leads to increase in fresh weight of roots. This result is verified by Hartmann et al., 1986 [3] .
The data collected in Table 2 goes with the trend and maximum (6.80 g) weight was observed in P 3 (polybag 7ʺx12ʺ) which was at par with (6.68 g) in P 4 (polybag 8ʺx12ʺ). The results obtained are similar with the findings of Riadh et al. (1995) [7] in Acacia auriculiformis and Chickrassia tabularis.
Table 2 shows the significant differences in fresh weight of shoots. Significantly maximum (35.41 g) weight was observed in P 3 (polybag 7ʺx12ʺ) which was followed by (31.20 g) in P 4 (polybag 8ʺx12ʺ).
Significantly maximum (13.64 g) weight was observed in P 3 (polybag 7ʺx12ʺ) which was followed by (12.25 g) in P 4 (polybag 8ʺx12ʺ).
Shoot to root ratio of fresh weight
Significantly maximum (2.16 g) weight was observed in P 3 (polybag 7ʺx12ʺ) which was followed by (1.93 g) in P 4 (polybag 8ʺx12ʺ).
Shoot to root ratio of dry weight
Maximum (2.80) ratio was observed in P 3 (polybag 7ʺx12ʺ) which was followed by (1.92) in P 4 (polybag 8ʺx12ʺ).
Conclusion
Production of good and healthy planting material depends on many factors but polybag size is one of the crucial factors which affect overall growth of rootstock which indirectly affect the development of scion.
On the basis of the study entitled "Influence of different budding techniques on budding success of Nagpur mandarin", 7ʺx12ʺ polybag size show promising results with respect to the growth factors like plant height, number of leaves and leaf area of rootstock and fresh weightdry weight of roots, fresh weight-dry weight of shoots, shoot to root ratio of fresh weight and dry weight of budded seedlings.
Acknowledgement
AcknowledgmentThe authors are highly grateful of the Department of Fruit Science, Faculty of Horticulture, Dr. Panjabrao Deshmukh Krishi Vidhyapeeth, Akola, Maharashtra for providing the necessary facilities to carry out the experiment work.
References
- Bose TK, Mitra SK, Sanyal D. Fruits: Tropical and Subtropical. Culcutta: Naya Uddyog; 2001. p. 109-225.
- Halandakar PM, Jadav BB. Softwood grafting of clove (Syzygium aromaticum) on jamun (Syzygium cumini) rootstock. Journal of Plantation Crops. 2001;29(3):4649.
- Hartmann HT, Kester DE. Plant Propagation: Principles and Practices. 4th ed. New Delhi: Prentice Hall of India Pvt. Ltd.; 1986. p. 410-411.
- Marler TE, Davies FT. Container size influences growth and development of young pecan seedlings. Journal of the American Society for Horticultural Science. 1987;112(2):207-211.
- NeSmith DS, Bridges DC, Barbour JC. Bell pepper responses to root restriction. Journal of Plant Nutrition. 1992;15:2763-2776.
- Panse VG, Sukhatme PV. Statistical Methods for Agricultural Workers. Rev. ed. Sukhatme PV, Amble VN, editors. New Delhi: ICAR; 1997. p. 97-156.
- Riadh SM, Hossain MK, Akhter S. Effect of polybag size on initial growth of Acacia auriculiformis and Chikrassia tabularis seedlings in the nurser;.1995.
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