Method development and validation of pregabalin in bulk and tablet dosage forms by UV spectroscopy

Abstract

A simple and sensitive UV-spectrophotometric method was developed and validated for the determination of pregabalin in bulk and pharmaceutical formulations. The estimation carried out by using 1.2 pH HCl buffer as solvent. The absorbance was measured at 210 nm. The method was linear in the range of 2-10 μg/ml with correlation coefficient value 0.997. The recovery was found to be 98.12-101.17%. The relative standard deviation was found to be less than 2. The method was validated with respect to accuracy, precision, assay, ruggedness, robustness, limit of detection and limit of quantitation. This is found to be simple, specific, precise, accurate, reproducible and low-cost UV-spectrophotometric method. The method can be useful for the day-to-day routine analysis in the quality control departments of bulk and pharmaceutical formulations industries.