Identification of true hybrid progenies in two different crosses of groundnut (Arachis hypogaea L.) involving TMV 2 as the female parent by using SSR markers

Abstract

In the present experiment hybridization programme was conducted to identify the best possible parents contrasting for late leaf spot disease resistance in groundnut. A collection of 250 minicore germplasm lines were screened for LLS disease resistance, among them four lines were selected as male parent manifested resistance for LLS disease, while TMV-2 and GKVK-4 are agronomically superior varieties but susceptible for LLS disease were used for hybridization programme. Among the crosses carried out in the hybridization best two crosses of groundnut namely TMV 2 × ICGV 86699 (C1) and TMV 2 × GBFDS 272 (C2) were selected. These crosses were identified with flanking markers associated with six QTLs and five QTLs respectively. Out of 53 selected SSR primers conferring 38 QTLs were identified from reported research papers. 12 primers and 10 primers were found polymorphic flanking marker for these two crosses. SSR markers provide more discriminate power and faster identification true hybrids. The complementary banding pattern were resolved on 3% (1.5% +1.5%) Metaphor agarose Gel. The cross C1 and C2 consisted of 100 and 210 F1 plants respectively, Out of these F1’s 23 and 46 and plants were confirmed as true hybrids using two highly polymorphic markers namely GM2301 and GM2246 for the cross C1 and TC6H03 and GM1760 used for cross C2. True hybrids were used for back cross breeding programme with recurrent parent TMV-2.