In silico analysis of FLP 18 gene of Anguina tritici and phylogenetic analysis with other nematodes

Abstract

flp 18is a neuropeptide coding gene of FMRFamide-like peptides family having important role in behavioural regulation such as locomotory, feeding and reproduction of nematodes. Genome annotation of the newly sequenced genome of seed gall nematode (Anguina tritici) has revealed the presence of flp 18 in their genome. flp 18 gene has been proven as effective target for management of Meloidogyne incognita. In view of importance of this gene here we analyse the significance of flp 18 gene in A. tritici in different nematode species through the In silico approach. Homologus nucleotide sequences in other nematode species for the gene flp 18 were obtained on performing nucleotide BLAST in WormBase Parasite database. Multiple sequence alignment, identity and phylogenetic analysis were performed on the retrived homologus sequences using CLUSTAL W programme. The BLAST result revealed the presence of flp 18 hits in different groups of plant parasitic nematodes with best hit (76) to Ditylenchus dipsaci. Based on the finding it has been suggested that the flp 18 of A. tritici is conserved with identical homologs, and might have functional similarity among different nematodes species.