Fraction of latex of Euphorbia umbellata induced inhibitory proliferation in triple negative breast tumor cell

Abstract

The objective of this study was to evaluate the antitumor potential of the acetate and chloroform fractions extracted from Euphorbia umbellatalat ex. The cytotoxicity of fractions was evaluated by the MTT colorimetric assay after 24 and 48h at concentrations ranging from 2-30 μg/mL, also the mitochondrial electrical potential by confocal microscopy. The cell cycle, the percentage of fragmented DNA, and the mitochondrial electrical potential were quantified by flow cytometry. Our results suggest that the cytotoxicity of fractions occurs due to the modulation of the apoptotic pathway, a fact that corroborates with compounds already identified from chemical alkaloids groups, with the fractions isolated by acetate and chloroform being those that presented better results in this and previous results antitumor potential new drugs developed by our group.