Testing of biocontrol efficacy of T. harzianum against virulent isolate of A. niger

Abstract

Among four isolates of A. niger viz., AN-I, AN-II, AN-III and AN-IV, the isolate AN-IV was found to be more virulent which gave higher mycelial growth after 48 hrs. (34.00 mm) in in vitro condition and caused much disease incidence (50.00%) under pot culture experiment. Protein molecular weights of isolates were estimated through SDS-PAGE. Isolate AN-III and AN-IV showed maximum three protein bands having molecular weight ranging from 29 kDa to 200 kDa. However, biocontrol efficacy of T. harzianum on virulent isolates of A. niger (AN-IV) showed effective inhibition in radial growth of A. niger, increased seed germination (91.43%) and caused least disease incidence (8.57%).