HPLC quantification of andrographolide in different parts of Andrographis paniculata (Burm.f.) Wall. ex Nees

Abstract

Andrographis paniculata, a member of family Acanthaceae commonly found in plains of India, Pakistan and Sri Lanka and is an important medicinal plant, which is used in diseases like fevers, jaundice, colic dysentery and dyspepsia and also act as hepatoprotective, bitter tonic, stomachic, immunostimulant, antidote, antimalarial agent etc. The medicinal value of the plant is mainly due to active components as andrographolide, deoxy-andrographolide, neoandrographolide etc. present in the species. In the present study andrographolide, a major diterpenoid in Andrographis paniculata was quantified in different parts of plant using HPLC. The leaves were found to contain the highest content of andrographolide. The content ranged from 0.054 – 4.686%. The other parts studied were stem, flowering tops and roots. The andrographolide was detected in all four parts of the plant.