Method validation for determination of commonly used fungicides in rice and husk by gas liquid chromatography: Tandem mass spectrometry

Abstract

The objective of this study is to establish and validate a modified quick, easy, cheap, effective, rugged and safe (QuEChERS) to analyze various fungicide (azoxystrobin, difenconazole, pencycuron, propiconazole, tebuconazole and trifloxystrobin) residues in rice. The six fungicides were initially extracted from rice samples using acetonitrile and water (2:1) followed by cleanup with the Primary Secondary Amine (PSA). Samples were concentrated and analyzed on gas chromatography–tandem mass spectrometry (GC–MS/MS) using MRM mode to achieve lower detection level. Targeted compounds were determined within 30.0 minutes showing the limits quantification (LOQ) 0.005 mgkg-1 for all the fungicides except pencycuron (0.01 mgkg-1) At the fortification level of 0.01–0.1 mg kg-1, the average recoveries ranged from 75.25 to 93.58% with RSD