In vitro regeneration and rapid multiplication of Kalanchoe blossfeldiana: An important ornamental plant

Abstract

In the present study, the effect of different auxins and cytokinins alone and in combinations were studied for in vitro regeneration of whole plant of Kalanchoe. At initiation stage, longest shoot (1.5 cm) was obtained on MS + 0.4 mg/l BAP while at multiplication, maximum number of shoots (8) were obtained on MS + 2.5 mg/l BAP using nodal explant. The greenish and higher mass of callus (56.6 mg per explants) was obtained on MS + 2 mg/l 2,4-D + 2 mg/l BAP. The maximum number of shoots (7) was observed when callus was grown on MS + BAP (0.2 mg/l) and NAA (0.1 mg/l). The best rooting response (7 roots) was observed on MS + 1mg/l IBA. The micro propagated plantlets established successfully in acclimatization medium containing soil:cocopeat (1:1).