Validation of hybrid seed production technology in rice under mid hill conditions and DNA fingerprinting of hybrids

Abstract

The present investigation entitled, “Validation of hybrid seed production technology in rice under mid hill conditions and DNA fingerprinting of hybrids” was undertaken to study the extent of hybridity in some public bred rice hybrids under mid hill conditions. The experimental material comprising of 4 rice hybrids Sahyadri 1, Sahyadri 2, Sahyadri 3 & Sahyadri 4 each with two parental lines (male R and female A) were evaluated in Randomized Block Design with 4 replications during the kharif 2015 at experimental farm of Rice and Wheat Research Centre (RWRC), CSKHPKV, Malan. Staggered planting of male lines was done and dates were decided on the basis of standard planting protocol of respective lines of hybrids. One planting was done on the date of standard protocol and other two dates were 5 days early and 5 days later to protocol. Data were recorded on various morphological traits and were analyzed using standard procedures. The study revealed that maximum hybridity % and hybrid seed yield per ha (q) was observed for Sahyadri 4 hybrid and Sahyadri 2 hybrid and it was minimum for Sahyadri 1 hybrid and Sahyadri 3 hybrid. The significant differences for germination percentage first & final count, accelerated ageing test (24, 48 & 72 hrs for first & final count), speed of germination, seedling dry weight, seed vigour index 1 & 2 indicated that the seeds of 4 hybrids have different storability capabilities. Moderate PCV and GCV (15-30%) was observed for hybridity percentage, accelerated ageing test (24 hrs for first count), accelerated ageing test (48 hrs for first & final count), accelerated ageing test (72 hrs for final count), speed of germination and seedling dry weight. High heritability was found for florets per panicle, seeds per panicle, hybridity percentage, 1000 seed weight, germination percentage first count, accelerated ageing test (24 & 48 hrs for first & final count), accelerated ageing test (72 hrs for first count), speed of germination, seedling dry weight and seed vigour 2. Furthermore, the hybridity of F1 crosses was confirmed at molecular level through SSR primers.