Biochemical studies on genotypic characterization of Pseudomonas fluorescens isolates by PCR-RAPD analysis

Abstract

Plant growth promoting rhizobacterial strains belonging to fluorescent Pseudomonads were isolated from the rhizosphere of citrus in Vidarbha region of Maharashtra, India. On the basis of morphological and biochemical studies, they were categorized as Pseudomonas fluorescens, P. putida and P. aurigonosa. Genotyping of these P. fluorescens strains was made by PCR–RAPD analysis since differentiation by biochemical methods was limited. Among the 16 primers screened, six primers (OPA-16, OPB-18, OPC-15, OPC-19, OPG-05 and OPG-10) were polymorphic showed better amplification of DNA while other were recorded monomorphic. Similarity co-efficient between each pair of accessions was used to construct a dendrogram using the unweighted paired group method with arithemetic average (UPGMA).