Biochemical and molecular studies on effect of polymer seed coating of soybean

Abstract

The present study was conducted at the Research Farm Adhartal, Department of Plant Breeding and Genetics, JNKVV, Jabalpur (M.P.) during Kharif season of 2016-17. Research experiment was laid out in a Randomized block design with five replications and four treatments i.e T0 No treatment or (Water + Thiram), T1- Polymer seed coating (Disco AG SP Red L-200) + Thiram + Carboxine, T2- Polymer seed coating (Disco AG SP Red L-200) + Thiram + Genius coat, T3- Polymer seed coating (Disco AG SP Red L-200) + Thiram + Mycorhiza comprised of soybean genotype JS JS 20-29. Five plants were randomly selected from each treatment and replications for Phenological and Physiological Parameters. Maximum protein % was registered in Treatments Polymer seed coating (Disco AG SP Red L-200) + Thiram + Genius coat (T2) (40.64), Treatments T3 (20.04) possessed the maximum fat % and showed differed significantly among them. The DNA amplification pattern revealed that a total number of 42 RAPD loci were amplified with an average of 7.0 loci per RAPD primer comprising 30 polymorphic loci (71.43%) and 12 monomorphic loci (28.57 %). The range of polymorphism was 50 % (OPA-01&OPA 02) to 89.0 % (OPA 07). Amplified product (band) size ranged from 300 bp (OPA-03) to 2800 bp (OPA-05). The no of bands ranged from 04 (OPA-02) to 09 (OPA -07).