Standardization the protocol for high genomic DNA yield and quality for guava cultivars

Abstract

DNA is the basic requirement for genomic analysis in plants and animals so its quality must be high for accurate result. DNA extraction in recalcitrant guava is difficult because of the high polyphenols, tannins and polysaccharides present in the guava leaf tissues. Therefore, a modified protocol is present here, which is improvement over the existing over the CTAB method. It involves double extraction using 3.5 % CTAB in the initial cell lysis, followed by RNase treatment. The highest amount of DNA per gram of fine ground leaf tissue was in L-49 extracted using modified CTAB method (485.2µg/ml of leaves) followed by Allahabad Safeda (398.1µg/ml of leaves) and Chinese guava (380.5 µg/ml of leaves). The superior quality DNA from thirteen guava cultivars and two species evident from the purity Indies. The absorbance ratio of 1.74 to 1.8 (A260/A280), was recorded which reflects the quality of isolated DNA.