In vitro evaluation of mungbean (Vigna radiata L.) genotypes under salinity stress conditions

Abstract

The present investigation was carried out at Department of Botany and Plant Physiology, Faculty of Basic Sciences and Humanities, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar) during 2016 to evaluate mungbean genotypes against salinity stress under in vitro condition. Twenty genotypes of mungbean viz. IPM 312-19, MH 560A, Pusa Vishal, Pusa 1602, SML 1781, SML 668, Pusa 1641, HDM 12, TMB 37, TMB 163, Pusa 1632, MH 1142, IPM- 99-125, Pusa 1502, SHIM 14-4, Pusa-1601, OUM 11-5, IPM 409-4, Pant M5 and SMP 16-12 were grown in vitro at different salinity levels. On the basis of physiological parameters viz., germination percent, seedling length, root length, shoot length and seedling dry weight contrasting set of genotypes were identified. It is found that with increasing salt stress the germination per cent declined this decline in germination per cent might be due to, salinity creating osmotic stress which prevents the water uptake and by toxic effects of ions on embryo viability as well as oxidative damage. The root length and shoot length also show same trends, this may be due to toxic effect of NaCl and decrease in turgor and cell elongation. The genotypes MH 560 A and IPM 312-19 were identified as relatively tolerant, whereas PUSA 1641and SML 668 were found susceptible genotype.