Evaluation of anti-arthritic, thrombolytic and cytotoxic activities of methanolic and ethanolic extract of Macaranga denticulata leaves

Abstract

For the evaluation of potential anti-arthritic, thrombolytic and cytotoxic activities of methanolic and ethanolic leaf extract of Macaranga denticulata, bovine serum albumin induced anti-arthritic method, clot lysis method and brine shrimp lethality bioassay was performed. In the case of protein denaturation, methanolic and ethanolic leaf extract of M. denticulata showed 68.40±0.92%* and 63.35±1.22%* whereas standard diclofenac sodium exhibited 90.58±0.82% at 1000 µg/ml (highest conc.) respectively. At lowest concentration (31.25 µg/ml), methanolic and ethanolic leaf extract demonstrated 26.31±0.31%* and 22.44±0.42%* whereas diclofenac sodium showed 51.75±0.92 respectively. Both methanolic and ethanolic leaf extract exhibited promising thrombolytic activity which were 38.23±0.88*% and 33.52±1.64**% respectively whereas standard streptokinase showed 63.54±2.61%**. Methanolic and ethanolic leaf extract of this plant showed significant cytotoxic activity. LC50 value of methanolic leaf extract was 74.30 µg/ml and ethanolic leaf extract was 97.77 µg/ml whereas 12.59 µg/ml cytotoxic effect was observed for the standard vincristine sulphate.