Protein profile of Native population of Antheraea assamensis Helfer (Muga Silkworm) of Assam, India

Abstract

The present study seeks to investigate the comparative estimation and electrophoretic profile of haemolymph proteins among the different semi domesticated morphs (green, blue, orange) and the wild (bivoltine) morph of Antheraea assamensis Helfer in order to investigate their differential gene expression. The proteomic approach was utilized to investigate the proteome of the haemolymph of early, mid and late stages of 5th instar (larval stage) and to improve the understanding of their important bioprocess and gene expression situation. The results suggest that the quantitative approach is useful to arrive at a conclusion of genetic variation among the morphs. SDS-PAGE electrophoresis of protein associated with haemolymph revealed separation of 16 distinct protein bands among which some of them represent the storage and immunity related proteins.