Anthocyanin production in calyx and callus of Roselle (Hibiscus sabdariffa L.) and its impact on antioxidant activity

Abstract

Objective: Identifiaction of anthocyanin froA¹ calyx and callus of Hibiscus sabdariffa and comparison of their antioxidant properties. Methods: HPLC fingerprints for different extracts were carried out using Agilent HPLC system. HPLC experiments were conducted using a reversed-phase C18 column. Antioxidant activity of anthocyanin was analyzed by using the 1,1-dipheny-2-picrylhydrazyl (DPPH). Results: The HPLC studies using mobile phase system for each fraction showed various peaks in each extract. Six anthocyanins were determined in callus with the major compounds were cyanidin3-O-sambubioside and delphinidin 3-O-glucoside. In calyx, four anthocyanins were determined with the major compounds were delphinidin 3-O-sambubioside and cyanidin-3-O-sambubioside. Antioxidant activity is higher with anthocyanin from callus than that of callus. Conclusion: Hibiscus sabdariffa is a potential source of natural antioxidants. In vitro culture has increased the number of anthocyanins; this shows interest of vitro methods in improving dietary and pharmacological qualities of Hibiscus anthocyanin.