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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers

Nucleic Acids Research · 1990 · Vol. 18(22) · pp. 6531–6535

John G. Williams✉Anne R. KubelikKenneth J. LivakJ. Antoni Rafalski(DuPont (United States))Scott Tingey(DuPont (United States))

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Abstract

Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.

Genomics and Phylogenetic Studies Chromosomal and Genetic Variations Genetic diversity and population structureBiologyRAPDGeneticsRestriction fragment length polymorphismDNAGenetic markerPolymorphism (computer science)Amplified fragment length polymorphismPolymerase chain reactionDNA sequencing

MeSH terms

Base CompositionBase SequenceZea maysCrossing Over, GeneticDNAGenetic MarkersHumansMolecular Sequence DataNeurospora crassaNucleotide MappingPolymorphism, Restriction Fragment LengthGlycine maxPolymerase Chain Reaction

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