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The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

Gene · 1982 · Vol. 19(3) · pp. 259–268
Jeffrey VieiraJoachim Messing
Advanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene DeliveryBiologyPlasmidRestriction enzymeRestriction siteMultiple cloning siteGeneticsCloning vectorMutagenesisKanamycinRestriction digest

MeSH terms

Escherichia coliBase SequenceCloning, MolecularDNA Transposable ElementsDNA Restriction EnzymesDNA, RecombinantDrug Resistance, MicrobialKanamycinMutationPlasmids

Funding

  • U.S. Department of Energy
  • University of Minnesota
  • University of California, Davis
Citations
6,131
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172.59
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42
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References
Genetic engineering. Principles and methods
Gene · 1987 · 707 citations
A new method for sequencing DNA.
Proceedings of the National Academy of Sciences · 1977 · 7,962 citations
DNA sequencing with chain-terminating inhibitors
Proceedings of the National Academy of Sciences · 1977 · 69,196 citations
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