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Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

Applied and Environmental Microbiology · 1998 · Vol. 64(2) · pp. 795–799

Julian R. Marchesi✉(University of Wales)Takuichi Sato(Niigata University)Andrew J. Weightman(University of Wales)Tracey A. Martin(University of Wales)John C. Fry(University of Wales)Sarah Hiom(University Of Bristol Dental Hospital)William G. Wade(Guy's Hospital)

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Abstract

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

Molecular Biology Techniques and Applications Bacteriophages and microbial interactions Genomics and Phylogenetic StudiesBiology16S ribosomal RNAGeneBacteriaGeneticsPolymerase chain reactionComputational biologyRibosomal RNABacterial geneticsMicrobiology