articleTop 1% cited

Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae

Journal of Antimicrobial Chemotherapy · 2010 · Vol. 65(3) · pp. 490–495

Caroline Dallenne(Sorbonne Université)Anaelle Da Costa(Sorbonne Université)Dominique Decré(Sorbonne Université)Christine F. Favier(Bio-Rad (France))Guillaume Arlet✉(Hôpital Tenon)

View via DOI Publisher page

Abstract

We report here the development of a useful method composed of a set of six multiplex PCRs and one simplex PCR for the rapid screening of the most frequently encountered beta-lactamases. This method allowed direct sequencing from the PCR products.

Antibiotic Resistance in Bacteria Enterobacteriaceae and Cronobacter Research Pharmaceutical and Antibiotic Environmental ImpactsEnterobacteriaceaeMultiplex polymerase chain reactionBiologyGeneMultiplexMicrobiologyEnterobacteriaceae InfectionsComputational biologyPolymerase chain reactionGenetics

MeSH terms

Anti-Bacterial Agentsbeta-LactamasesDNA, BacterialEnterobacteriaceaeEnterobacteriaceae InfectionsHumansMicrobial Sensitivity TestsMolecular Sequence DataSensitivity and SpecificityPolymerase Chain ReactionSequence Analysis, DNADNA Primersbeta-Lactam Resistancebeta-Lactams

Citations

1,645

FWCI

17.89

field-weighted impact

References

Percentile

100%

vs. same field & year

Citations per year

Cited by

Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global Dimensions