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Analysis of Circulating MicroRNA: Preanalytical and Analytical Challenges
Clinical Chemistry · 2011 · Vol. 57(6) · pp. 833–840
Jennifer S. McDonaldDragana MiloševićHoney V. Reddi(Mayo Clinic in Arizona)Stefan K. GrebeAlicia Algeciras‐Schimnich✉
Abstract
Detailed validation of the preanalytical steps affecting miRNA detection and quantification is critical when considering the use of individual miRNAs as clinical biomarkers. Unless these causes of imprecision are considered and mitigated, only miRNAs that are extremely up- or downregulated will be suitable as clinical biomarkers.
MicroRNA in disease regulationRNA Interference and Gene DeliveryExtracellular vesicles in diseasemicroRNAHemolysisCoefficient of variationComputational biologyBiologyBioinformaticsChemistryImmunologyChromatographyGene
MeSH terms
FemaleHemolysisHumansMaleSensitivity and SpecificityReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA StabilityMicroRNAs
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References
Identification of Tissue-Specific MicroRNAs from Mouse
Current Biology · 2002 · 3,292 citations
Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers
Nature Cell Biology · 2008 · 4,954 citations
RETRACTED: MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer
Gynecologic Oncology · 2008 · 2,297 citations
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
Nature Cell Biology · 2007 · 12,686 citations
MicroRNA expression in response to murine myocardial infarction: miR-21 regulates fibroblast metalloprotease-2 via phosphatase and tensin homologue
Cardiovascular Research · 2009 · 611 citations
A novel and universal method for microRNA RT-qPCR data normalization
Genome biology · 2009 · 1,026 citations
Detection of microRNA Expression in Human Peripheral Blood Microvesicles
PLoS ONE · 2008 · 1,439 citations
Circulating microRNAs as stable blood-based markers for cancer detection
Proceedings of the National Academy of Sciences · 2008 · 7,894 citations
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