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International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
PLoS neglected tropical diseases · 2011 · Vol. 5(1) · pp. e931–e931
Alejandro G. Schijman✉(Experimental Medicine and Biology Institute)Margarita Bisio(Consejo Nacional de Investigaciones Científicas y Técnicas)Liliana Orellana(University of Buenos Aires)Mariela Sued(Instituto Cardiovascular de Buenos Aires)Tomás Duffy(Consejo Nacional de Investigaciones Científicas y Técnicas)Ana María Mejía‐Jaramillo(Universidad de Antioquia)Carolina Cura(Experimental Medicine and Biology Institute)Frederic Auter(Société Nationale des Chemins de Fer Français (France))Vincent Véron(University of French Guiana)Yvonne Qvarnström(Centers for Disease Control and Prevention)Stijn Deborggraeve(Instituut voor Tropische Geneeskunde)Gisely Hijar(Instituto Nacional de Salud)Inés Zulantay(University of Chile)Raúl Horacio Lucero(National University of the Chaco Austral)Elsa Velázquez(National Institute of Industrial Technology)Tatiana Tellez(Universidad Mayor)Zunilda Sanchez Leon(Universidad Nacional de Asunción)Lúcia Maria da Cunha Galvão(Faculdade de Medicina de Petrópolis)Debbie Nolder(London School of Hygiene & Tropical Medicine)María M. Monje Rumi(National University of Salta)José Eduardo Levi(Hospital Sírio-Libanês)Juan David Ramírez(Universidad de Los Andes)Pilar Zorrilla(Institut Pasteur de Montevideo)María Flóres-Chávez(Centro Nacional de Epidemiología)Maria I. Jercic(Instituto de Salud Pública de Chile)Gladys Crisante(University of the Andes)Néstor Áñez(University of the Andes)Ana Maria de Castro(Universidade Federal de Goiás)Clara Isabel González(Industrial University of Santander)Karla Y. Acosta-Viana(Secretaría de Investigación, Innovación y Educación Superior)Pedro Yachelini(National University of Santiago del Estero)Faustino Torrico(Universidad Mayor)Carlos Robello(Institut Pasteur de Montevideo)Patricio Diosque(National University of Salta)Omar Triana Chávez(Universidad de Antioquia)Christine Aznar(University of French Guiana)Graciela Russomando(Universidad Nacional de Asunción)Philippe Büscher(Instituut voor Tropische Geneeskunde)Azzedine Assal(Société de Réanimation de Langue Française)Felipe Guhl(Universidad de Los Andes)Sérgio Sosa Estani(Administración Nacional de Laboratorios e Institutos de Salud)Alexandre J. da Silva(Centers for Disease Control and Prevention)Constança Britto(Fundação Oswaldo Cruz)Alejandro O. Luquetti(National Council for Scientific and Technological Development)Janis Ladzins(World Health Organization)
Abstract
This study represents a first crucial step towards international validation of PCR procedures for detection of T. cruzi in human blood samples.
Trypanosoma species research and implicationsInsect symbiosis and bacterial influencesResearch on Leishmaniasis StudiesDNA extractionMinicircleBiologyTrypanosoma cruziPolymerase chain reactionChagas diseaseTriatomaReal-time polymerase chain reactionDNAKinetoplast
MeSH terms
HumansInternational CooperationParasitologySensitivity and SpecificityTrypanosoma cruziChagas DiseaseDNA, ProtozoanPolymerase Chain Reaction
Funding
- United Nations
- World Health Organization
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Pan American Health Organization
Citations
472
FWCI
23.35
field-weighted impact
References
56
Percentile
100%
vs. same field & year
Citations per year
References
Accurate Real-Time PCR Strategy for Monitoring Bloodstream Parasitic Loads in Chagas Disease Patients
PLoS neglected tropical diseases · 2009 · 336 citations
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