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Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages fromEscherichia coli933

FEMS Microbiology Letters · 1987 · Vol. 44(1) · pp. 109–114

M P Jackson(Uniformed Services University of the Health Sciences)Roger Neill(Walter Reed Army Institute of Research)Alison D. O’Brien(Uniformed Services University of the Health Sciences)Randall K. Holmes(Uniformed Services University of the Health Sciences)John W. Newland✉(Walter Reed Army Institute of Research)

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Abstract

The nucleotide sequence of the Shiga-like toxin type II (SLT-II) structural genes cloned from bacteriophage 933W of the enterohemorrhagic Escherichia coli O157:H7 strain 933 was determined. This sequence was compared with the published sequence for the structural genes of the antigenically distinct Shiga-like toxin type I (SLT-I) encoded by bacteriophage 933J. The SLT-I and SLT-II structural genes shared 58% overall nucleotide and 56% amino acid sequence homologies. The A and B subunits of SLT-I and SLT-II were nearly identical in size and had similar secondary structures and hydropathy plots. The regulation proposed for the SLT-II operon is similar to that previously proposed for SLT-I.

Bacteriophages and microbial interactions Escherichia coli research studies Viral gastroenteritis research and epidemiologyOperonEscherichia coliBiologyNucleic acid sequenceBacteriophageGeneShiga toxinShiga-like toxinStructural geneGenetics