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Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Gene · 1988 · Vol. 67(1) · pp. 21–30

Chu di Guana(New England Biolabs (United States))Ping Lib(Temple University)Paul D. Riggsa✉(New England Biolabs (United States))Hiroshi Inouyeb(Temple University)

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Bacterial Genetics and Biotechnology RNA and protein synthesis mechanisms Bacteriophages and microbial interactionsMaltose-binding proteinPeriplasmic spaceBiologyEscherichia coliFusion proteinBiochemistryMaltoseSignal peptideExpression vectorBinding protein

MeSH terms

Alkaline PhosphataseBase SequenceCarrier ProteinsCloning, MolecularDNA Restriction EnzymesEscherichia coliGenesGenetic VectorsGenotypeMembrane ProteinsMolecular Sequence DataMonosaccharide Transport ProteinsRecombinant Fusion ProteinsRecombinant ProteinsATP-Binding Cassette Transporters

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Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems