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Determination of DNA base composition by reversed-phase high-performance liquid chromatography
FEMS Microbiology Letters · 1984 · Vol. 25(1) · pp. 125–128
Jin Támaoka✉(The University of Tokyo)Kazuo Komagata(The University of Tokyo)
Abstract
DNA base composition was determined by reversed-phase high-performance liquid chromatography (HPLC). DNA was hydrolysed into nucleosides with nuclease P1 and bacterial alkaline phosphatase. The mixture of nucleosides was applied to HPLC without any further purification. One determination by chromatography needed 2 µg of hydrolysed nucleosides and took only 8 min. The relative standard error of nucleoside analysis was less than 1%. The system described here gives a direct and precise method for determining DNA base composition.
DNA and Nucleic Acid ChemistryPorphyrin Metabolism and DisordersDNA Repair MechanismsChromatographyHigh-performance liquid chromatographyNucleaseChemistryNucleosideDNAHydrolysisReversed-phase chromatographyComposition (language)Base (topology)
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References
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature
Journal of Molecular Biology · 1962 · 4,101 citations
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl
Journal of Molecular Biology · 1962 · 1,592 citations
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