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A method for the absolute quantification of cDNA using real-time PCR

Journal of Immunological Methods · 2003 · Vol. 278(1-2) · pp. 261–269

Joseph A. Whelan(St George's, University of London)Nick B Russell(St George's Hospital)Michael Whelan✉(St George's, University of London)

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Molecular Biology Techniques and Applications Virus-based gene therapy research RNA Interference and Gene DeliveryComplementary DNAReal-time polymerase chain reactionStandard curveMolecular biologyPlasmidBiologyComputational biologyPolymerase chain reactionCalibrationDNA

MeSH terms

Fluorescent DyesHumansLymphocytesOrganic ChemicalsPlasmidsReference StandardsRNA, MessengerSensitivity and SpecificityCytokinesDNA PrimersDNA, ComplementaryImmunomagnetic SeparationReverse Transcriptase Polymerase Chain ReactionGene Expression Profiling

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