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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

Proceedings of the National Academy of Sciences · 1987 · Vol. 84(21) · pp. 7413–7417

Philip L. Felgner✉(Institute of Organic Chemistry)Thomas R. Gadek(Institute of Organic Chemistry)Mai Marie Holm(Institute of Organic Chemistry)R. M. Roman(Institute of Organic Chemistry)Hardy Chan(Institute of Organic Chemistry)M Wenz(Institute of Organic Chemistry)Jeffrey P. Northrop(Institute of Organic Chemistry)G M Ringold(Institute of Organic Chemistry)Mark Danielsen(Institute of Organic Chemistry)

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Abstract

A DNA-transfection protocol has been developed that makes use of a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). Small unilamellar liposomes containing DOTMA interact spontaneously with DNA to form lipid-DNA complexes with 100% entrapment of the DNA, DOTMA facilitates fusion of the complex with the plasma membrane of tissue culture cells, resulting in both uptake and expression of the DNA. The technique is simple, highly reproducible, and effective for both transient and stable expression of transfected DNA. Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.

RNA Interference and Gene Delivery Viral Infectious Diseases and Gene Expression in Insects RNA and protein synthesis mechanismsTransfectionLiposomeDNACationic liposomeChemistryCell biologyMolecular biologyBiochemistryBiophysicsBiology

MeSH terms

Quaternary Ammonium CompoundsAnimalsCalcium PhosphatesCell LineDEAE-DextranGenetic EngineeringIndicators and ReagentsL CellsLiposomesSimian virus 40Transfection

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Liposomes in drug delivery: Progress and limitations